PDF(Pubmed)
Abstract：
Nasopharyngeal carcinoma (NPC) is the most prevalent head and neck cancer in Indonesia, with 100% Epstein-Barr virus (EBV) infection in tumor cells. NPC is rare in the Netherlands. The involvement of EBV in NPC pathogenesis is reflected by early onset aberrant IgA antibody responses to various EBV proteins. Screening for elevated EBV-IgA levels is proposed for NPC risk assessment in endemic countries but is poorly studied in nonendemic regions. This study analyzed the overall diversity (immunoblot) as well as the prevalence and normalized levels of IgA responses to immunodominant peptide epitopes of EBV proteins VCA P18, EBNA 1, and Zebra (Zta) (N-terminus, P 125, P 130, full-length recombinant Zebra) in Indonesian (n=50) and Dutch (n=50) patients with NPC. The results confirmed that elevated levels of IgA-VCA P18 and IgA-EBNA 1 were found in both NPC populations, but that IgA-Zta was more variable. IgA-Zta responses were more pronounced in Indonesian NPC cases, reflecting more frequent EBV reactivation overall. IgA-VCA P18 and IgA-EBNA are independent tumor markers and are both necessary for NPC risk assessment. Overall, these results confirmed the diagnostic benefit of combined IgA-VCA P18/-EBNA 1 testing for NPC risk assessment in endemic and nonendemic populations.
摘要：
鼻咽癌（NPC）是印度尼西亚最常见的头颈癌，在肿瘤细胞中具有100%EB病毒(EBV)感染。NPC在荷兰很少见。EBV在NPC发病机理中的参与反映为对各种EBV蛋白的早发性异常IgA抗体应答。在流行国家中，建议对升高的EBV-IgA水平进行筛查以进行NPC风险评估，但在非流行地区研究甚少。这项研究分析了整体多样性（免疫印迹）以及对EBV蛋白VCAP18，EBNA1和斑马（Zta）（N端，P125，P130，全长重组斑马）在印度尼西亚（n=50）和荷兰（n=50）NPC患者中。结果证实，在两个NPC人群中均发现IgA-VCAP18和IgA-EBNA1水平升高，但是IgA-Zta的变化更大。IgA-Zta反应在印度尼西亚NPC病例中更为明显，反映总体上更频繁的EBV再激活。IgA-VCAP18和IgA-EBNA是独立的肿瘤标志物，都是NPC风险评估所必需的。总的来说,这些结果证实了IgA-VCAP18/-EBNA1联合检测对地方性和非地方性人群NPC风险评估的诊断益处.
