PDF(Pubmed)
Abstract：
Following the implementation of the European regulation limiting the presence of tropane alkaloids in certain foods, a survey was conducted in Serbia on 103 maize products (grits, polenta and semolina) to determine atropine and scopolamine content using liquid chromatography with tandem-mass spectrometry analysis (LC-MS/MS). The probability of exceeding the Acute Reference Dose (ARfD; 0.016 μg/kg bw/day) of the sum of atropine and scopolamine by consuming these products was tested. Overall, across age categories - children, younger and older adolescents, and adults, the group ARfD was exceeded by 21.4%, 17.5%, 11.7% and 11.7% of the samples, with maximum exposure reaching as much as 19-, 13-, 9- and 9-fold the group ARfD, respectively. Nevertheless, polenta could be the most favorable dietary option (17.9% of positive samples, 7.7% resulting in excessive exposure in children, reaching a maximum of 1.4-fold the group ARfD). According to the reported findings, adverse health effects of tropane alkaloids cannot be ruled out. The Margin of Exposure, founded on a clinically significant acute effects dose established by FAO/WHO, ranged from 1194 to 2381 (mean) and from 28 to 56 (95th percentile) across age categories. These estimates should certainly draw the attention of food authorities and nutritionist, particularly in the case of highly sensitive populations with contraindications and high consumers of corn products, such as coeliac patients.
摘要：
在欧洲法规实施后，限制某些食品中托烷生物碱的存在，在塞尔维亚对103种玉米产品进行了调查(粗粒，玉米粥和粗面粉）使用液相色谱和串联质谱分析（LC-MS/MS）确定阿托品和东pol碱含量。测试了通过食用这些产品超过阿托品和东莨菪碱总和的急性参考剂量(ARfD;0.016μg/kg体重/天)的概率。总的来说,跨年龄段-儿童，年轻和年长的青少年，和成年人，ARfD组超过21.4%，17.5%,11.7%和11.7%的样品，最大暴露达到19-，13-,ARfD组的9倍和9倍，分别。然而，玉米粥可能是最有利的饮食选择(17.9%的阳性样本，7.7%导致儿童过度接触，达到ARfD组的最大1.4倍）。根据报告的发现，不能排除托烷生物碱对健康的不良影响。曝光的边际,建立在粮农组织/世卫组织确立的临床显著急性效应剂量基础上，年龄类别的范围为1194至2381（平均值）和28至56（第95百分位数）。这些估计当然应该引起食品当局和营养学家的注意，特别是在有禁忌症和玉米产品高消费者的高度敏感人群中，如乳糜泻患者。
