Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique.

使用病毒载体追踪研究成年小鼠基底神经节中投影特异性回路的方案 ，光遗传学,和膜片钳技术。

• 发表时间: 2023 Sep 15
• 来源期刊: STAR Protoc
• DOI: 10.1016/j.xpro.2023.102551
• PMID: 37660296
• 文章类型: Journal Article
• 作者列表: Ji YW,Xu XY,Yin C,Zhou C,Xiao C

关键词: Model Organisms; Molecular Biology; Molecular/Chemical Probes; Neuroscience

Mesh: Animals; Mice; Optogenetics / methods; Patch-Clamp Techniques; Basal Ganglia

Abstract

Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell- and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze synaptic function of these circuits utilizing a patch-clamp technique. This protocol is applicable to probe potential circuit targets for treatment of brain diseases. For complete details on the use and execution of this protocol, please refer to Ji et al.1.

摘要

突触强度和可塑性的分析提供了复杂神经回路的功能见解。这里,我们描述了使用顺行和逆行病毒载体对不同的基底神经节回路进行细胞和投影特异性光遗传学操作的步骤。我们利用膜片钳技术定量分析了这些电路的突触功能。该协议适用于用于治疗脑疾病的探针潜在电路靶标。有关此协议的使用和执行的完整详细信息，PleaserefertoJietal.1.