Abstract：
Developing cell cryopreservation methods on chips is not only crucial for biomedical science but also represents an innovative approach for preserving traditional cell samples. This study presents a simple method for direct cell freezing and thawing on chip, allowing for long-term storage of cells. During the freezing process, cells were injected into the microchannel along with a conventional cell cryopreservation solution, and the chip was packed using a self-sealing bag containing isopropyl alcohol and then stored in a -80°C refrigerator until needed. During the thawing process, microcolumn arrays with a spacing of 8 µm were strategically incorporated into the microfluidic chip design to effectively inhibit cells from the channel. The breast cancer cell lines MDA-MB-231 and B47D demonstrated successful thawing and growth after cryopreservation for 1 month to 1 year. These findings offer a direct cell freezing and thawing method on a microfluidic chip for subsequent experiments.
摘要：
在芯片上开发细胞冷冻保存方法不仅对生物医学科学至关重要，而且代表了保存传统细胞样品的创新方法。本研究提出了一种在芯片上直接冻融细胞的简单方法，允许细胞的长期储存。在冻结过程中,将细胞与常规细胞冷冻保存溶液一起注入微通道，使用含有异丙醇的自密封袋包装芯片,然后储存在-80°C冰箱中直至需要。在解冻过程中,将间距为8μm的微柱阵列策略性地并入微流体芯片设计中,以有效地抑制来自通道的细胞。乳腺癌细胞系MDA-MB-231和B47D在冷冻保存一个月至一年后显示出成功的解冻和生长。这些发现为后续实验提供了在微流控芯片上的直接细胞冷冻和解冻方法。
