METHODS: The expression level of NLRC4, NLRP1, ASC, and CASPASE-1 within PBMCs isolated from all RA subjects by quantitative real-time PCR. GAPDH gene was used as a reference gene. Measurement of serum level of IL-1β and IL-18 was performed using ELISA.
RESULTS: Results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease.
CONCLUSIONS: Understanding the role of inflammasomes in RA pathogenesis helps in finding promising therapy for the treatment and management of this disease.
方法:NLRC4、NLRP1、ASC、和通过定量实时PCR从所有RA受试者分离的PBMC内的CASPASE-1。GAPDH基因用作参考基因。使用ELISA进行血清IL-1β和IL-18水平的测量。
结果:结果显示炎症小体表达失调,可能参与疾病炎症过程的发病机理。
结论:了解炎症小体在RA发病机制中的作用有助于寻找治疗和管理该疾病的有希望的疗法。