PDF(Pubmed)
Abstract：
OBJECTIVE: This study aims to demonstrate the role of some inflammasomes genes: NLRC4 (the NLR family, CARD domain-containing protein 4), NLRP1 (NLR family, pyrin domain-containing 1), ASC (Apoptosis-associated speck-like protein containing a CARD), and CASPASE-1 in the pathogenesis of Rheumatoid arthritis (RA) in Egyptian population.
METHODS: The expression level of NLRC4, NLRP1, ASC, and CASPASE-1 within PBMCs isolated from all RA subjects by quantitative real-time PCR. GAPDH gene was used as a reference gene. Measurement of serum level of IL-1β and IL-18 was performed using ELISA.
RESULTS: Results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease.
CONCLUSIONS: Understanding the role of inflammasomes in RA pathogenesis helps in finding promising therapy for the treatment and management of this disease.
METHODS: The expression level of NLRC4, NLRP1, ASC, and CASPASE-1 within PBMCs isolated from all RA subjects by quantitative real-time PCR. GAPDH gene was used as a reference gene. Measurement of serum level of IL-1β and IL-18 was performed using ELISA.
RESULTS: Results showed dysregulated inflammasomes expression that may participate in the pathogenesis of the inflammatory process of the disease.
CONCLUSIONS: Understanding the role of inflammasomes in RA pathogenesis helps in finding promising therapy for the treatment and management of this disease.
摘要：
目的：本研究旨在证明一些炎性体基因的作用：NLRC4（NLR家族，含有CARD结构域的蛋白质4），NLRP1（NLR系列，含pyrin结构域1)，ASC（含有CARD的凋亡相关斑点样蛋白），和CASPASE-1在埃及人群类风湿关节炎（RA）的发病机制中。
方法：NLRC4、NLRP1、ASC、和通过定量实时PCR从所有RA受试者分离的PBMC内的CASPASE-1。GAPDH基因用作参考基因。使用ELISA进行血清IL-1β和IL-18水平的测量。
结果：结果显示炎症小体表达失调，可能参与疾病炎症过程的发病机理。
结论：了解炎症小体在RA发病机制中的作用有助于寻找治疗和管理该疾病的有希望的疗法。
方法：NLRC4、NLRP1、ASC、和通过定量实时PCR从所有RA受试者分离的PBMC内的CASPASE-1。GAPDH基因用作参考基因。使用ELISA进行血清IL-1β和IL-18水平的测量。
结果：结果显示炎症小体表达失调，可能参与疾病炎症过程的发病机理。
结论：了解炎症小体在RA发病机制中的作用有助于寻找治疗和管理该疾病的有希望的疗法。
