PDF(Pubmed)
Abstract：
Sepsis is one of the possible outcomes of severe trauma, and it poses a dire threat to human life, particularly in immunocompromised people. The most prevalent pathogens are bacteria and fungi, but viruses should not be overlooked. For viral metagenomic analysis, we collected blood samples from eight patients with post-traumatic sepsis before and seven days after treatment. The results demonstrated that Anellovirus predominated the viral community, followed by Siphoviridae and Myoviridae, and that the variations in viral community and viral load before and after treatment were not statistically significant. This study allows us to investigate methods for establishing NGS-based viral diagnostic instruments for detecting viral infections in the blood of sepsis patients so that antiviral therapy can be administered quickly.
摘要：
脓毒症是严重创伤的可能后果之一,它对人类生命构成了可怕的威胁，特别是在免疫功能低下的人群中。最常见的病原体是细菌和真菌，但是病毒不应该被忽视。对于病毒宏基因组分析，我们在治疗前和治疗后7天收集了8例创伤后脓毒症患者的血样.结果表明Anellovirus在病毒群落中占主导地位，其次是虹彩病毒科和Myoviridae，治疗前后病毒群落和病毒载量的变化无统计学意义。这项研究使我们能够研究建立基于NGS的病毒诊断仪器的方法，以检测败血症患者血液中的病毒感染，从而可以快速进行抗病毒治疗。
