Abstract：
An assay for the measurement of myeloperoxidase (Mpx) in porcine saliva was developed and validated, and factors influencing Mpx and another two biomarkers of inflammation and immune system, the protein S100A12 and the inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), were studied. The spectrophotometric method for Mpx measurement validated in this assay showed an adequate analytical performance including precision and accuracy. When a group of twenty healthy pigs was sampled every 4 h from 8 a.m. until 8 p.m., Mpx and S100A12 showed significant increases at 4 p.m., whereas ITIH4 concentration showed a significant decrease at 12 a.m. Increases were also seen in salivary Mpx, S100A12, and ITIH4 levels 24 h after the intramuscular administration of Escherichia coli lipopolysaccharide in five pigs; whereas in a non-septic inflammation after the subcutaneous administration of turpentine oil to five pigs changes were seen in S100A12 at 3 h and in ITIH4 at 48 h. When a stressful situation consisting of the transportation and stay of 4 h to a slaughterhouse of 24 pigs was performed, all analytes were increased after 4 h of lairage in the slaughterhouse compared with the values that were obtained the day before at the same time of the day. Mpx can be measured in the saliva of pigs with the automated assay described in this report. Mpx, S100A12, and ITIH4 salivary levels can change depending on the hour of the day in which the sample is taken, and increases can be produced due to sepsis, non-septic inflammation and stress.
摘要：
开发并验证了猪唾液中髓过氧化物酶（Mpx）的测定方法，以及影响Mpx和另外两种炎症和免疫系统生物标志物的因素，蛋白质S100A12和α-胰蛋白酶抑制剂重链4（ITIH4），被研究过。在该测定中验证的Mpx测量的分光光度法显示出足够的分析性能，包括精密度和准确性。当一组二十只健康猪从上午八时至晚上八时每四小时取样,Mpx和S100A12在下午4点显示显着增加，而ITIH4浓度显示在上午12点显著下降，唾液Mpx也增加，S100A12和ITIH4在5头猪肌内给药大肠杆菌脂多糖后24小时的水平；而在非感染性炎症中，对5头猪皮下给药松节油后，在3小时的S100A12和48小时的ITIH4中看到了变化。当进行压力情况时，包括运输和停留4小时到24头猪的屠宰场，与前一天在当天同一时间获得的值相比，所有分析物在屠宰场中进行4小时后都增加了。Mpx可以通过本报告中描述的自动化测定法在猪的唾液中进行测量。Mpx,S100A12和ITIH4唾液水平可以根据取样的时间而变化，并且可以由于败血症而产生增加，非感染性炎症和压力。
