PDF(Pubmed)
Abstract：
From the safety inside vehicles, Knowsley Safari offers visitors a close-up encounter with captive olive baboons. As exiting vehicles may be contaminated with baboon stool, a comprehensive coprological inspection was conducted to address public health concerns. Baboon stools were obtained from vehicles, and sleeping areas, inclusive of video analysis of baboon–vehicle interactions. A purposely selected 4-day sampling period enabled comparative inspections of 2662 vehicles, with a total of 669 baboon stools examined (371 from vehicles and 298 from sleeping areas). As informed by our pilot study, front-line diagnostic methods were: QUIK-CHEK rapid diagnostic test (RDT) (Giardia and Cryptosporidium), Kato–Katz coproscopy (Trichuris) and charcoal culture (Strongyloides). Some 13.9% of vehicles were contaminated with baboon stool. Prevalence of giardiasis was 37.4% while cryptosporidiosis was <0.01%, however, an absence of faecal cysts by quality control coproscopy, alongside lower than the expected levels of Giardia-specific DNA, judged RDT results as misleading, grossly overestimating prevalence. Prevalence of trichuriasis was 48.0% and strongyloidiasis was 13.7%, a first report of Strongyloides fuelleborni in UK. We advise regular blanket administration(s) of anthelminthics to the colony, exploring pour-on formulations, thereafter, smaller-scale indicator surveys would be adequate.
摘要：
从车内的安全来看，KnowsleySafari为游客提供了与圈养的橄榄狒狒的近距离接触。因为离开的车辆可能被狒狒凳污染，为解决公共卫生问题，进行了全面的协同检查。狒狒凳子是从车辆上获得的，和睡眠区，包括狒狒-车辆互动的视频分析。故意选择的4天采样期，可以对2662辆汽车进行比较检查，共检查了669个狒狒凳子(371个来自车辆，298个来自睡眠区)。根据我们的试点研究，一线诊断方法是：QUIK-CHEK快速诊断测试（RDT）（贾第鞭毛虫和隐孢子虫），Kato-Katz共镜（Trichuris）和木炭培养（Strongyloides）。约有13.9％的车辆被狒狒粪便污染。贾第鞭毛虫病的患病率为37.4%，而隐孢子虫病的患病率<0.01%，然而,通过质量控制共检没有粪便囊肿，同时低于预期的贾第虫特异性DNA水平，判定RDT结果具有误导性，严重高估了患病率。旋毛虫病患病率为48.0%,线虫病患病率为13.7%,英国Strongloidesfuelleborni的第一份报告。我们建议定期对殖民地进行驱虫药的全面管理，探索浇灌配方，此后,较小规模的指标调查就足够了。
