PDF(Pubmed)
Abstract：
UNASSIGNED: Luminal A and B subtypes of breast cancer (BC) comprises up to 70% of all BC patients. LncRNAs can affect many biological and pathological processes, and dysregulation of them is related to human cancers. The potential role of lncRNA LINC00968 in luminal BC is still unclear.
UNASSIGNED: We analyzed the LINC00968 expression across 44 paired luminal BC tissues from the TCGA-BRCA RNA sequencing dataset. Besides, we used the GEPIA2 web server and GENEVESTIGATOR software, as well. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) assay was performed to confirm the LINC00968 expression in 71 paired luminal BC tissues and two luminal A cell lines (MCF7 and T47D). Moreover, to better understanding the potential role of LINC00968 in luminal BC, computational data analyses including co-expression analysis, functional annotation analysis, and genetic alteration analysis have been done.
UNASSIGNED: The results of data analyses retrieved from BRCA dataset and databases revealed the significant downregulation of LINC00968 in luminal A and B BC. Also, the results of qRT-PCR in luminal BC tissues and cell lines confirmed the earlier data. LINC00968 expression was negatively associated with tumor stage and lymph node metastasis. Additionally, functional annotation analyses revealed that LINC00968 might be involved in vascular development and angiogenesis, extracellular matrix organization, and cell motility and migration. LINC00968 might play role in some cancer-related signaling pathways.
UNASSIGNED: Our study found that downregulation of LINC00968 might promote tumorigenesis, invasion, and metastasis of luminal BC.
UNASSIGNED: We analyzed the LINC00968 expression across 44 paired luminal BC tissues from the TCGA-BRCA RNA sequencing dataset. Besides, we used the GEPIA2 web server and GENEVESTIGATOR software, as well. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) assay was performed to confirm the LINC00968 expression in 71 paired luminal BC tissues and two luminal A cell lines (MCF7 and T47D). Moreover, to better understanding the potential role of LINC00968 in luminal BC, computational data analyses including co-expression analysis, functional annotation analysis, and genetic alteration analysis have been done.
UNASSIGNED: The results of data analyses retrieved from BRCA dataset and databases revealed the significant downregulation of LINC00968 in luminal A and B BC. Also, the results of qRT-PCR in luminal BC tissues and cell lines confirmed the earlier data. LINC00968 expression was negatively associated with tumor stage and lymph node metastasis. Additionally, functional annotation analyses revealed that LINC00968 might be involved in vascular development and angiogenesis, extracellular matrix organization, and cell motility and migration. LINC00968 might play role in some cancer-related signaling pathways.
UNASSIGNED: Our study found that downregulation of LINC00968 might promote tumorigenesis, invasion, and metastasis of luminal BC.
摘要：
■乳腺癌（BC）的管腔A和B亚型占所有BC患者的70％。LncRNAs可以影响许多生物学和病理过程，它们的失调与人类癌症有关。lncRNALINC00968在管腔BC中的潜在作用尚不清楚。
我们分析了来自TCGA-BRCARNA测序数据集的44个配对腔BC组织的LINC00968表达。此外,我们使用了GEPIA2网络服务器和GENEVESTIGATOR软件，也是。进行实时定量逆转录PCR(qRT-PCR)测定以确认在71个配对的腔BC组织和两个腔A细胞系(MCF7和T47D)中的LINC00968表达。此外，为了更好地理解LINC00968在管腔BC中的潜在作用，计算数据分析，包括共表达分析，功能注释分析，并进行了遗传改变分析。
■从BRCA数据集和数据库检索的数据分析结果显示，LINC00968在管腔A和BCB中的显着下调。此外，腔BC组织和细胞系中的qRT-PCR结果证实了较早的数据。LINC00968的表达与肿瘤分期和淋巴结转移呈负相关。此外,功能注释分析显示LINC00968可能参与血管发育和血管生成，细胞外基质组织，和细胞运动和迁移。LINC00968可能在一些癌症相关的信号通路中发挥作用。
■我们的研究发现，LINC00968的下调可能会促进肿瘤发生，入侵,管腔BC转移。
我们分析了来自TCGA-BRCARNA测序数据集的44个配对腔BC组织的LINC00968表达。此外,我们使用了GEPIA2网络服务器和GENEVESTIGATOR软件，也是。进行实时定量逆转录PCR(qRT-PCR)测定以确认在71个配对的腔BC组织和两个腔A细胞系(MCF7和T47D)中的LINC00968表达。此外，为了更好地理解LINC00968在管腔BC中的潜在作用，计算数据分析，包括共表达分析，功能注释分析，并进行了遗传改变分析。
■从BRCA数据集和数据库检索的数据分析结果显示，LINC00968在管腔A和BCB中的显着下调。此外，腔BC组织和细胞系中的qRT-PCR结果证实了较早的数据。LINC00968的表达与肿瘤分期和淋巴结转移呈负相关。此外,功能注释分析显示LINC00968可能参与血管发育和血管生成，细胞外基质组织，和细胞运动和迁移。LINC00968可能在一些癌症相关的信号通路中发挥作用。
■我们的研究发现，LINC00968的下调可能会促进肿瘤发生，入侵,管腔BC转移。
