PDF(Pubmed)
Abstract：
The presence of Staphylococcus aureus, a normal human flora on cellphones of different professionals in Ile-Ife was investigated with a view to determining their antibiotic susceptibility profile and nature of resistance and virulence genes. One hundred swab samples were collected aseptically from mobile phones of various users based on their profession. Surfaces of the mobile phones were swabbed and the streak plate method was used to isolate colonies showing characteristic golden yellow on mannitol salt agar plates. These isolates were further identified using standard microbiological methods. The antibiotic susceptibility of the isolates was determined using Kirby-Bauer\'s disk diffusion technique. Molecular detection of nuc, mecA and pvl genes in some isolates was carried out by polymerase chain reaction technique. All the 36 isolates obtained in this study were 100% resistant to amoxicillin and augmentin; the isolates also displayed 55.6%, 44.4% and 41.7% resistance to ceftriazone, erythromycin and chloramphenicol, respectively. Based on resistance to oxacillin, prevalence of methicillin resistant Staphylococcus aureus (MRSA) was 11.1%. Only one S. aureus was positive for plasmid analysis. MecA gene was genetically confirmed in four (4) out of the 16 suspected phenotypic MRSA strains, nuc gene was confirmed in all 28 isolates investigated, while there was no pvl gene in the strains investigated. Mobile phones harbor multiple antibiotics resistant S. aureus, which are responsible for important diseases in humans and could be difficult to manage with antibiotics thereby posing serious health risks.
摘要：
金黄色葡萄球菌的存在，研究了Ile-Ife不同专业人员的手机上的正常人类菌群，以确定其抗生素敏感性以及抗性和毒力基因的性质。根据他们的职业，从各种用户的手机中无菌收集了一百个拭子样本。擦拭手机的表面，并使用条纹平板法分离在甘露醇盐琼脂平板上显示特征性金黄色的菌落。使用标准微生物学方法进一步鉴定这些分离物。使用Kirby-Bauer的圆盘扩散技术确定分离株的抗生素敏感性。nuc的分子检测，通过聚合酶链反应技术在一些分离物中进行mecA和pvl基因。在这项研究中获得的所有36个分离株对阿莫西林和增强素具有100％的耐药性；这些分离株还显示55.6％，对头孢曲酮的耐药率为44.4%和41.7%，红霉素和氯霉素，分别。基于对苯唑西林的耐药性，耐甲氧西林金黄色葡萄球菌(MRSA)的患病率为11.1%.只有一个金黄色葡萄球菌对质粒分析是阳性的。MecA基因在16个疑似表型MRSA菌株中的四（4）个中被遗传证实，在所有28个被调查的分离株中确认了nuc基因，而研究的菌株中没有pvl基因。手机含有多种抗生素抗性金黄色葡萄球菌，它们是人类重要疾病的原因，并且可能难以用抗生素进行管理，从而构成严重的健康风险。
