Forty participants who received ovarian stimulation using a GnRH antagonist protocol in their first in vitro fertilization treatment were recruited. From each patient, two follicular samples (one preovulatory follicle, > 18 mm; one mid-antral follicle, < 14 mm) were collected without flushing during oocyte retrieval. In total, 80 FF samples were collected from 40 patients. The expression profiles of angiogenesis-related proteins in FF were analyzed via Luminex high-performance assays. Recorded patient data included antral follicle count, anti-müllerian hormone, age, and BMI. Serum samples were collected on menstrual cycle day 2, the trigger day, and the day of oocyte retrieval. Hormone concentrations including day 2 FSH/LH/E2/P4, trigger day E2/LH/P4, and retrieval day E2/LH/P4 were measured by chemiluminescence assay.
Ten angiogenic factors were highly expressed in FF: eotaxin, Gro-α, IL-8, IP-10, MCP-1, MIG, PAI-1 (Serpin), VEGF-A, CXCL-6, and HGF. The concentrations of eotaxin, IL-8, MCP1, PAI-1, and VEGF-A were significantly higher in preovulatory follicles than those in mid-antral follicles, while the Gro-α and CXCL-6 expressional levels were lower in preovulatory than in mid-antral follicles (p < 0.05). Logistic regression and receiver operating characteristic (ROC) analysis revealed that VEGF-A, eotaxin, and CXCL-6 were the three strongest predictors of oocyte maturity. The combination of VEGF-A and CXCL-6 predicted oocyte maturity with a higher sensitivity (91.7%) and specificity (72.7%) than other combinations.
Our findings suggest that VEGF-A, eotaxin, and CXCL-6 concentrations in FF strongly correlate with oocyte maturity from the mid-antral to preovulatory stage. The combination of VEGF-A and CXCL-6 exhibits a relatively good prediction rate of oocyte maturity during in vitro fertilization.
■招募了40名在首次体外受精治疗中使用GnRH拮抗剂方案进行卵巢刺激的参与者。每个病人,两个卵泡样本(一个排卵前卵泡,>18毫米;一个中窦卵泡,<14mm)在卵母细胞取出过程中没有潮红的情况下收集。总的来说,从40名患者收集了80份FF样品。通过Luminex高性能测定分析FF中血管生成相关蛋白的表达谱。记录的患者数据包括窦卵泡计数,抗苗勒管激素,年龄,BMI。在月经周期第2天,触发日收集血清样本,和取卵日。通过化学发光法测量激素浓度,包括第2天的FSH/LH/E2/P4,触发天的E2/LH/P4和取回天的E2/LH/P4。
■10种血管生成因子在FF中高表达:eotaxin,Gro-α,IL-8,IP-10,MCP-1,MIG,PAI-1(Serpin),VEGF-A,CXCL-6和HGF。eotaxin的浓度,IL-8、MCP1、PAI-1、VEGF-A在排卵前卵泡明显高于中腔卵泡,排卵前卵泡中Gro-α和CXCL-6的表达水平低于中腔卵泡(p<0.05)。Logistic回归和受试者工作特征(ROC)分析显示,VEGF-A,eotaxin,和CXCL-6是卵母细胞成熟度的三个最强预测因子。VEGF-A和CXCL-6的组合比其他组合以更高的灵敏度(91.7%)和特异性(72.7%)预测卵母细胞成熟度。
■我们的研究结果表明VEGF-A,eotaxin,FF中的CXCL-6浓度与从窦中期到排卵前期的卵母细胞成熟度密切相关。VEGF-A和CXCL-6的组合在体外受精过程中显示出相对较好的卵母细胞成熟度预测率。