Abstract：
BACKGROUND: Belamcanda chinensis (L.) DC. (BC) and Iris tectorum Maxim. (ITM) have been widely used in recent years due to their remarkable curative effects on sore throat, cough and asthma. but they are often misused due to their similar appearance. A comprehensive comparison of the chemical composition, biological activity, pharmacokinetics and tissue distribution between the two active differential components has not been performed. Differences in their specific effects have not been fully elucidated.
OBJECTIVE: This work aims at differentiating between BC and ITM in terms of appearance, chemical composition, biological activity, pharmacokinetics and tissue distribution.
METHODS: In this study, the HPLC-FP method was used to find the differences between the chemical components of BC and ITM. The pharmacological experiments were used to compare the differences in activity, including in vitro anti-inflammatory activity with LPS-induced inflammation model of RAW 264.7 cells, inhibition of AChE activity, and the regulation of isolated small intestinal smooth muscle in mice. The pharmacokinetic and tissue distribution profiles were used to analyze the differences between the two in rats.
RESULTS: The types of isoflavones in BC and ITM are basically the same, but their contents in ITM is much higher than that in BC. At the same doses, the release of TNF-α, NO, IL-1β and IL-6 from RAW 264.7 cells in the ITM group was lower than that of the BC group, and the in vitro anti-inflammatory activity of ITM was stronger than that of BC. Meanwhile, ITM had stronger inhibition ability to inhibit AChE activity than BC. The BC extract exhibited an inhibitory effect on the isolated small intestinal smooth muscle of mice, and the ITM extract showed stimulatory effect at low concentration and inhibitory effect at high concentration. There were significant differences in drug-time profiles, kinetic parameters and tissue distribution.
CONCLUSIONS: There are significant differences in the multidimensional aspects of appearance, chemical composition, biological activity, pharmacokinetics, and tissue distribution between BC and ITM. This study provides a theoretical basis for the quality control, pharmacological efficacy and clinical application of the two herbs.
OBJECTIVE: This work aims at differentiating between BC and ITM in terms of appearance, chemical composition, biological activity, pharmacokinetics and tissue distribution.
METHODS: In this study, the HPLC-FP method was used to find the differences between the chemical components of BC and ITM. The pharmacological experiments were used to compare the differences in activity, including in vitro anti-inflammatory activity with LPS-induced inflammation model of RAW 264.7 cells, inhibition of AChE activity, and the regulation of isolated small intestinal smooth muscle in mice. The pharmacokinetic and tissue distribution profiles were used to analyze the differences between the two in rats.
RESULTS: The types of isoflavones in BC and ITM are basically the same, but their contents in ITM is much higher than that in BC. At the same doses, the release of TNF-α, NO, IL-1β and IL-6 from RAW 264.7 cells in the ITM group was lower than that of the BC group, and the in vitro anti-inflammatory activity of ITM was stronger than that of BC. Meanwhile, ITM had stronger inhibition ability to inhibit AChE activity than BC. The BC extract exhibited an inhibitory effect on the isolated small intestinal smooth muscle of mice, and the ITM extract showed stimulatory effect at low concentration and inhibitory effect at high concentration. There were significant differences in drug-time profiles, kinetic parameters and tissue distribution.
CONCLUSIONS: There are significant differences in the multidimensional aspects of appearance, chemical composition, biological activity, pharmacokinetics, and tissue distribution between BC and ITM. This study provides a theoretical basis for the quality control, pharmacological efficacy and clinical application of the two herbs.
摘要：
背景：百事可乐（L.)DC。（BC）和IristectorumMaxim。(ITM)因其对喉咙痛的显著疗效,近年来得到了广泛的应用,咳嗽和哮喘.但是由于它们的外观相似，它们经常被滥用。综合比较化学成分,生物活性,尚未进行两种活性差异成分之间的药代动力学和组织分布。其具体作用的差异尚未完全阐明。
目的：这项工作旨在从外观上区分BC和ITM，化学成分,生物活性,药代动力学和组织分布。
方法：在本研究中，采用HPLC-FP法测定BC和ITM的化学成分差异。药理实验用于比较活性的差异，包括LPS诱导的RAW264.7细胞炎症模型的体外抗炎活性，抑制AChE活性，以及小鼠离体小肠平滑肌的调节。使用药代动力学和组织分布谱来分析两者之间在大鼠中的差异。
结果：BC和ITM中的异黄酮种类基本相同，但它们在ITM中的含量远高于BC。在相同的剂量下，TNF-α的释放，NO,ITM组RAW264.7细胞的IL-1β和IL-6低于BC组,ITM的体外抗炎活性强于BC。同时,ITM对AChE活性的抑制能力强于BC。BC提取物对小鼠离体小肠平滑肌有抑制作用,ITM提取物在低浓度下具有刺激作用，在高浓度下具有抑制作用。药物时间分布有显著差异，动力学参数和组织分布。
结论：外观的多维方面存在显着差异，化学成分,生物活性,药代动力学，BC和ITM之间的组织分布。本研究为质量控制提供了理论依据,两种中药的药理功效和临床应用。
目的：这项工作旨在从外观上区分BC和ITM，化学成分,生物活性,药代动力学和组织分布。
方法：在本研究中，采用HPLC-FP法测定BC和ITM的化学成分差异。药理实验用于比较活性的差异，包括LPS诱导的RAW264.7细胞炎症模型的体外抗炎活性，抑制AChE活性，以及小鼠离体小肠平滑肌的调节。使用药代动力学和组织分布谱来分析两者之间在大鼠中的差异。
结果：BC和ITM中的异黄酮种类基本相同，但它们在ITM中的含量远高于BC。在相同的剂量下，TNF-α的释放，NO,ITM组RAW264.7细胞的IL-1β和IL-6低于BC组,ITM的体外抗炎活性强于BC。同时,ITM对AChE活性的抑制能力强于BC。BC提取物对小鼠离体小肠平滑肌有抑制作用,ITM提取物在低浓度下具有刺激作用，在高浓度下具有抑制作用。药物时间分布有显著差异，动力学参数和组织分布。
结论：外观的多维方面存在显着差异，化学成分,生物活性,药代动力学，BC和ITM之间的组织分布。本研究为质量控制提供了理论依据,两种中药的药理功效和临床应用。
