Abstract：
Organelles are dynamic entities whose functions are essential for the optimum functioning of cells. It is now known that the juxtaposition of organellar membranes is essential for the exchange of metabolites and their communication. These functional apposition sites are termed membrane contact sites. Dynamic membrane contact sites between various sub-cellular structures such as mitochondria, endoplasmic reticulum, peroxisomes, Golgi apparatus, lysosomes, lipid droplets, plasma membrane, endosomes, etc. have been reported in various model systems. The burgeoning area of research on membrane contact sites has witnessed several manuscripts in recent years that identified the contact sites and components involved. Several methods have been developed to identify, measure and analyze the membrane contact sites. In this manuscript, we aim to discuss important methods developed to date that are used to study membrane contact sites.
摘要：
细胞器是动态实体，其功能对于细胞的最佳功能至关重要。现在已知，细胞器膜的并置对于代谢物的交换及其通讯至关重要。这些功能性并置位点称为膜接触位点。各种亚细胞结构如线粒体之间的动态膜接触位点，内质网，过氧化物酶体,高尔基体,溶酶体,脂滴，质膜，内体，等。已经在各种模型系统中得到了报道。近年来，膜接触部位研究的新兴领域见证了一些手稿，这些手稿确定了所涉及的接触部位和组件。已经开发了几种方法来识别，测量和分析膜接触部位。在这份手稿中,我们旨在讨论迄今为止开发的用于研究膜接触位点的重要方法。
