PDF(Pubmed)
Abstract：
A microbial fuel cell (MFC) biosensor with an anode as a sensing element is often unreliable at low or significantly fluctuating organic matter concentrations. To remove this limitation, this work demonstrates capillary action-aided carbon source delivery to an anode-sensing MFC biosensor for use in carbon-depleted environments, e.g., potable water. First, different carbon source delivery configurations using several thread types, silk, nylon, cotton, and polyester, are evaluated. Silk thread was determined to be the most suitable material for passive delivery of a 40 g L-1 acetate solution. This carbon source delivery system was then incorporated into the design of an MFC biosensor for real-time detection of toxicity spikes in tap water, providing an organic matter concentration of 56 ± 15 mg L-1. The biosensor was subsequently able to detect spikes of toxicants such as chlorine, formaldehyde, mercury, and cyanobacterial microcystins. The 16S sequencing results demonstrated the proliferation of Desulfatirhabdium (10.7% of the total population), Pelobacter (10.3%), and Geobacter (10.2%) genera. Overall, this work shows that the proposed approach can be used to achieve real-time toxicant detection by MFC biosensors in carbon-depleted environments.
摘要：
具有阳极作为感测元件的微生物燃料电池(MFC)生物传感器在低或显著波动的有机物质浓度下通常是不可靠的。要消除此限制，这项工作展示了毛细管作用辅助碳源输送到阳极传感MFC生物传感器，用于在贫碳环境中使用，例如，饮用水。首先,使用几种螺纹类型的不同碳源输送配置，丝绸,尼龙,棉花,和聚酯，进行了评估。丝线被确定为最适合被动递送40gL-1乙酸盐溶液的材料。然后将该碳源输送系统纳入MFC生物传感器的设计中，用于实时检测自来水中的毒性尖峰，提供56±15mgL-1的有机物浓度。生物传感器随后能够检测到毒物的峰值，例如氯，甲醛,水银,和蓝藻微囊藻毒素.16S测序结果证明了Desulfatirhibdium的增殖（占总人口的10.7％），Pelobacter(10.3%),和Geobacter（10.2％）属。总的来说,这项工作表明，所提出的方法可用于MFC生物传感器在贫碳环境中实时检测毒物。
