PDF(Pubmed)
Abstract：
Large White pigs are extensively utilized in China for their remarkable characteristics of rapid growth and the high proportion of lean meat. The economic traits of pigs, comprising reproductive and meat quality traits, play a vital role in swine production. In this study, 2295 individuals, representing three different genetic backgrounds Large White pig populations were used: 500 from the Canadian line, 295 from the Danish line, and 1500 from the American line. The GeneSeek 50K GGP porcine HD array was employed to genotype the three pig populations. Firstly, genomic selective signature regions were identified using the pairwise fixation index (FST) and locus-specific branch length (LSBL). By applying a top 1% threshold for both parameters, a total of 888 candidate selective windows were identified, harbouring 1571 genes. Secondly, the investigation of regions of homozygosity (ROH) was performed utilizing the PLINK software. In total, 25 genomic regions exhibiting a high frequency of ROHs were detected, leading to the identification of 1216 genes. Finally, the identified potential functional genes from candidate genomic regions were annotated, and several important candidate genes associated with reproductive traits (ADCYAP1, U2, U6, CETN1, Thoc1, Usp14, GREB1L, FGF12) and meat quality traits (MiR-133, PLEKHO1, LPIN2, SHANK2, FLVCR1, MYL4, SFRP1, miR-486, MYH3, STYX) were identified. The findings of this study provide valuable insights into the genetic basis of economic traits in Large White pigs and may have potential use in future pig breeding programs.
摘要：
大型白猪以其快速生长和瘦肉比例高的显着特征在中国被广泛使用。猪的经济性状,包括生殖和肉质性状，在养猪生产中起着至关重要的作用。在这项研究中,2295个人，代表三种不同的遗传背景使用了大型白猪种群：来自加拿大品系的500只，295来自丹麦线，和1500来自美国线。使用GeneSeek50KGGP猪HD阵列对三个猪群体进行基因分型。首先,使用成对固定指数(FST)和基因座特异性分支长度(LSBL)鉴定基因组选择性特征区.通过对这两个参数应用前1%的阈值，总共确定了888个候选选择窗口，有1571个基因.其次,纯合性区域(ROH)的调查使用PLINK软件进行.总的来说,检测到25个表现出高频率ROHs的基因组区域，导致1216个基因的鉴定。最后,从候选基因组区域中识别出的潜在功能基因进行了注释，和几个与生殖性状相关的重要候选基因(ADCYAP1、U2、U6、CETN1、Thoc1、Usp14、GREB1L、FGF12）和肉质性状（MiR-133，PLEKHO1，LPIN2，SHANK2，FLVCR1，MYL4，SFRP1，miR-486，MYH3，STYX）进行了鉴定。这项研究的结果为大型白猪经济性状的遗传基础提供了有价值的见解，并可能在未来的猪育种计划中具有潜在的用途。
