PDF(Pubmed)
Abstract：
Smoking has been linked to male infertility by affecting the sperm epigenome and genome. In this study, we aimed to determine possible changes in the transcript levels of PGAM5 (the phosphoglycerate mutase family member 5), PTPRN2 (protein tyrosine phosphatase, N2-type receptor), and TYRO3 (tyrosine protein kinase receptor) in heavy smokers compared to non-smokers, and to investigate their association with the fundamental sperm parameters. In total, 118 sperm samples (63 heavy-smokers (G1) and 55 non-smokers (G2)) were included in this study. A semen analysis was performed according to the WHO guidelines. After a total RNA extraction, RT-PCR was used to quantify the transcript levels of the studied genes. In G1, a significant decrease in the standard semen parameters in comparison to the non-smokers was shown (p < 0.05). Moreover, PGAM5 and PTPRN2 were differentially expressed (p ≤ 0.03 and p ≤ 0.01, respectively) and downregulated in the spermatozoa of G1 compared to G2. In contrast, no difference was observed for TYRO3 (p ≤ 0.3). In G1, the mRNA expression level of the studied genes was correlated negatively with motility, sperm count, normal form, vitality, and sperm membrane integrity (p < 0.05). Therefore, smoking may affect gene expression and male fertility by altering the DNA methylation patterns in the genes associated with fertility and sperm quality, including PGAM5, PTPRN2, and TYRO3.
摘要：
吸烟通过影响精子表观基因组和基因组与男性不育有关。在这项研究中,我们旨在确定PGAM5（磷酸甘油酸变位酶家族成员5）转录水平的可能变化，PTPRN2（蛋白酪氨酸磷酸酶，N2型受体），和TYRO3（酪氨酸蛋白激酶受体）在重度吸烟者与非吸烟者相比，并研究它们与基本精子参数的关系。总的来说,本研究包括118个精子样本（63个重度吸烟者（G1）和55个非吸烟者（G2））。根据WHO指南进行精液分析。总RNA提取后，RT-PCR用于定量所研究基因的转录水平。在G1中，与非吸烟者相比，标准精液参数显着降低（p<0.05）。此外，与G2相比，PGAM5和PTPRN2在G1的精子中差异表达（分别为p≤0.03和p≤0.01）并下调。相比之下,TYRO3无差异(p≤0.3).在G1中，研究基因的mRNA表达水平与运动呈负相关，精子计数,正常形式,活力,和精子膜完整性(p<0.05)。因此,吸烟可能通过改变与生育力和精子质量相关的基因中的DNA甲基化模式来影响基因表达和男性生育力。包括PGAM5、PTPRN2和TYRO3。
