PDF(Pubmed)
Abstract：
Salmonella Isangi is an infrequent serovar that has recently been reported in several countries due to nosocomial infections. A considerable number of reports indicate Salmonella Isangi multidrug resistance, especially to cephalosporins, which could potentially pose a risk to public health worldwide. Genomic analysis is an excellent tool for monitoring the emergence of microorganisms and related factors. In this context, the aim of this study was to carry out a genomic analysis of Salmonella Isangi isolated from poultry in Brazil, and to compare it with the available genomes from the Pathogen Detection database and Sequence Read Archive. A total of 142 genomes isolated from 11 different countries were investigated. A broad distribution of extended-spectrum beta-lactamase (ESBL) genes was identified in the Salmonella Isangi genomes examined (blaCTX-M-15, blaCTX-M-2, blaDHA-1, blaNDM-1, blaOXA-10, blaOXA-1, blaOXA-48, blaSCO-1, blaSHV-5, blaTEM-131, blaTEM-1B), primarily in South Africa. Resistome analysis revealed predicted resistance to aminoglycoside, sulfonamide, macrolide, tetracycline, trimethoprim, phenicol, chloramphenicol, and quaternary ammonium. Additionally, PMQR (plasmid-mediated quinolone resistance) genes qnr19, qnrB1, and qnrS1 were identified, along with point mutations in the genes gyrAD87N, gyrAS83F, and gyrBS464F, which confer resistance to ciprofloxacin and nalidixic acid. With regard to plasmids, we identified 17 different incompatibility groups, including IncC, Col(pHAD28), IncHI2, IncHI2A, IncM2, ColpVC, Col(Ye4449), Col156, IncR, IncI1(Alpha), IncFIB (pTU3), Col(B5512), IncQ1, IncL, IncN, IncFIB(pHCM2), and IncFIB (pN55391). Phylogenetic analysis revealed five clusters grouped by sequence type and antimicrobial gene distribution. The study highlights the need for monitoring rare serovars that may become emergent due to multidrug resistance.
摘要：
Isangi沙门氏菌是一种罕见的血清变型，由于医院感染，最近在几个国家有报道。相当多的报告表明沙门菌Isangi多药耐药，尤其是头孢菌素,这可能会对全球公共卫生构成潜在风险。基因组分析是监测微生物和相关因素出现的极好工具。在这种情况下，这项研究的目的是对从巴西家禽中分离出的沙门菌进行基因组分析，并将其与病原体检测数据库和序列读取存档中的可用基因组进行比较。研究了从11个不同国家分离的142个基因组。在检查的沙门菌基因组中鉴定出广谱β-内酰胺酶（ESBL）基因的分布（blaCTX-M-15，blaCTX-M-2，blaDHA-1，blaNDM-1，blaOXA-10，blaOXA-1，blaOXA-48，blaSCO-1，blashIV-131，TEM-5，主要在南非。抗性组分析显示预测对氨基糖苷的抗性，磺酰胺,大环内酯,四环素,甲氧苄啶,苯酚,氯霉素,和季铵。此外,鉴定了PMQR（质粒介导的喹诺酮耐药）基因qnr19，qnrB1和qnrS1，随着基因gyrAD87N的点突变，gyrAS83F,和gyrBS464F，赋予对环丙沙星和萘啶酸的抗性。关于质粒，我们确定了17个不同的不相容组，包括IncC,Col(pHAD28),IncHI2,IncHI2A,IncM2,ColpVC,Col(Ye4449),Col156,IncR,IncI1(Alpha),IncFIB(pTU3),Col(B5512),IncQ1,IncL,IncN,IncFIB(pHCM2),和IncFIB(pN55391)。系统发育分析揭示了按序列类型和抗菌基因分布分组的五个簇。该研究强调需要监测由于多药耐药性而可能出现的稀有血清变型。
