PDF(Pubmed)
Abstract：
The Eurasian beaver (Castor fiber) has been reintroduced successfully in Germany since the 1990s. Since wildlife is an important source of zoonotic infectious diseases, monitoring of invasive and reintroduced species is crucial with respect to the One Health approach. Three Eurasian beavers were found dead in the German federal states of Bavaria, North Rhine-Westphalia and Baden-Wuerttemberg in 2015, 2021 and 2022, respectively. During post-mortem examinations, Corynebacterium (C.) ulcerans could be isolated from the abscesses of two beavers and from the lungs of one of the animals. Identification of the bacterial isolates at the species level was carried out by spectroscopic analysis using MALDI-TOF MS, FT-IR and biochemical profiles and were verified by molecular analysis based on 16-23S internal transcribed spacer (ITS) region sequencing. Molecular characterization of the C. ulcerans isolates using whole-genome sequencing (WGS) revealed a genome size of about 2.5 Mbp and a GC content of 53.4%. Multilocus sequence typing (MLST) analysis classified all three isolates as the sequence type ST-332. A minimum spanning tree (MST) based on cgMLST allelic profiles, including 1211 core genes of the sequenced C. ulcerans isolates, showed that the beaver-derived isolates clearly group on the branch of C. ulcerans with the closest relationship to each other, in close similarity to an isolate from a dog. Antibiotic susceptibility testing revealed resistance to clindamycin and, in one strain, to erythromycin according to EUCAST, while all isolates were susceptible to the other antimicrobials tested.
摘要：
自1990年代以来，欧亚海狸（Castor纤维）已在德国成功重新引入。由于野生动物是人畜共患传染病的重要来源，监测入侵和重新引入的物种对于“一个健康”方法至关重要。在德国联邦巴伐利亚州发现三只欧亚海狸死亡，北莱茵-威斯特法伦州和巴登-符腾堡州分别于2015年、2021年和2022年。验尸时,棒状杆菌(C.）可以从两只海狸的脓肿和其中一只动物的肺中分离出溃疡。使用MALDI-TOFMS通过光谱分析在物种水平上进行细菌分离株的鉴定，FT-IR和生化谱，并通过基于16-23S内部转录间隔区（ITS）区域测序的分子分析进行验证。使用全基因组测序(WGS)对溃疡梭菌分离物的分子表征揭示了约2.5Mbp的基因组大小和53.4%的GC含量。多位点序列分型(MLST)分析将所有三个分离株分类为序列类型ST-332。基于cgMLST等位基因的最小生成树(MST)，包括1211个核心基因的测序的C.溃疡分离株，表明，海狸衍生的分离株清楚地聚集在溃疡的分支上，彼此关系最密切，与狗的分离非常相似。抗生素敏感性测试显示对克林霉素和，在一个菌株中，根据EUCAST的红霉素，而所有分离株都对其他测试的抗菌药物敏感。
