PDF(Pubmed)
Abstract：
The aim of this study was to assess the release profile of components in five different honeys (a New Zealand Manuka and two Western Australian honeys, a Jarrah honey and a Coastal Peppermint honey) and their corresponding honey-loaded gel formulations using a custom-designed Franz-type diffusion cell in combination with High-Performance Thin-Layer Chromatography (HPTLC). To validate the suitability of the customised setup, release data using this new approach were compared with data obtained using a commercial Franz cell apparatus, which is an established analytical tool to monitor the release of active ingredients from topical semisolid products. The release profiles of active compounds from pure honey and honey-loaded formulations were found to be comparable in both types of Franz cells. For example, when released either from pure honey or its corresponding pre-gel formulation, the percentage release of two Jarrah honey constituents, represented by distinct bands at RF 0.21 and 0.53 and as analysed by HPTLC, was not significantly different (p = 0.9986) at 12 h with over 99% of these honey constituents being released in both apparatus. Compared to the commercial Franz diffusion cell, the customised Franz cell offers several advantages, including easy and convenient sample application, the requirement of only small sample quantities, a large diffusion surface area, an ability to analyse 20 samples in a single experiment, and lower cost compared to purchasing a commercial Franz cell. Thus, the newly developed approach coupled with HPTLC is conducive to monitor the release profile of minor honey constituents from pure honeys and honey-loaded semisolid formulations and might also be applicable to other complex natural-product-based products.
摘要：
这项研究的目的是评估五种不同蜂蜜（一种新西兰麦卢卡和两种西澳大利亚蜂蜜，Jarrah蜂蜜和沿海薄荷蜂蜜）及其相应的载有蜂蜜的凝胶配方，使用定制设计的Franz型扩散池与高效薄层色谱法（HPTLC）相结合。要验证自定义设置的适用性，使用这种新方法的发布数据与使用商业弗朗兹细胞设备获得的数据进行了比较，这是一个既定的分析工具，用于监测活性成分从局部半固体产品的释放。发现在两种类型的Franz细胞中，纯蜂蜜和载有蜂蜜的制剂中活性化合物的释放曲线相当。例如,当从纯蜂蜜或其相应的预凝胶制剂中释放时，两种Jarrah蜂蜜成分的释放百分比，由RF0.21和0.53处的不同波段表示，并由HPTLC分析，在12小时时没有显着差异（p=0.9986），两个设备中都释放了超过99％的蜂蜜成分。与商用Franz扩散池相比，定制的弗朗兹细胞提供了几个优点，包括简单方便的示例应用程序，只有少量样品的要求，大的扩散表面积，在一个实验中分析20个样品的能力，与购买商业弗朗兹电池相比，成本更低。因此，新开发的方法与HPTLC联用有助于监测纯蜂蜜和载有蜂蜜的半固体制剂中次要蜂蜜成分的释放情况，也可能适用于其他复杂的基于天然产品的产品。
