PDF(Pubmed)
Abstract：
CONCLUSIONS: The development of an imaging technique to accurately identify biofilm regions on tissues and in wounds is crucial for the implementation of precise surface-based treatments, leading to better patient outcomes and reduced chances of infection.
OBJECTIVE: The goal of this study was to develop an imaging technique that relies on selective trypan blue (TB) staining of dead cells, necrotic tissues, and bacterial biofilms, to identify biofilm regions on tissues and wounds.
METHODS: The study explored combinations of ambient multi-colored LED lights to obtain maximum differentiation between stained biofilm regions and the underlying chicken tissue or glass substrate during image acquisition. The TB imaging results were then visually and statistically compared to fluorescence images using a shape similarity measure.
RESULTS: The comparisons between the proposed TB staining method and the fluorescence standard used to detect biofilms on tissues and glass substrates showed up to 97 percent similarity, suggesting that the TB staining method is a promising technique for identifying biofilm regions.
CONCLUSIONS: The TB staining method demonstrates significant potential as an effective imaging technique for the identification of fluorescing and non-fluorescing biofilms on tissues and in wounds. This approach could lead to improved precision in surface-based treatments and better patient outcomes.
OBJECTIVE: The goal of this study was to develop an imaging technique that relies on selective trypan blue (TB) staining of dead cells, necrotic tissues, and bacterial biofilms, to identify biofilm regions on tissues and wounds.
METHODS: The study explored combinations of ambient multi-colored LED lights to obtain maximum differentiation between stained biofilm regions and the underlying chicken tissue or glass substrate during image acquisition. The TB imaging results were then visually and statistically compared to fluorescence images using a shape similarity measure.
RESULTS: The comparisons between the proposed TB staining method and the fluorescence standard used to detect biofilms on tissues and glass substrates showed up to 97 percent similarity, suggesting that the TB staining method is a promising technique for identifying biofilm regions.
CONCLUSIONS: The TB staining method demonstrates significant potential as an effective imaging technique for the identification of fluorescing and non-fluorescing biofilms on tissues and in wounds. This approach could lead to improved precision in surface-based treatments and better patient outcomes.
摘要：
结论：准确识别组织和伤口中生物膜区域的成像技术的发展对于实施精确的基于表面的治疗至关重要，导致更好的患者结果和减少感染的机会。
目的：本研究的目的是开发一种依赖于死细胞的选择性锥虫蓝（TB）染色的成像技术，坏死组织，和细菌生物膜，识别组织和伤口上的生物膜区域。
方法：该研究探索了环境多色LED灯的组合，以在图像采集过程中获得染色的生物膜区域与下面的鸡组织或玻璃基材之间的最大差异。然后使用形状相似性测量将TB成像结果与荧光图像进行视觉和统计比较。
结果：拟议的TB染色方法与用于检测组织和玻璃基材上生物膜的荧光标准品之间的比较显示高达97％的相似性，表明TB染色方法是一种有前途的识别生物膜区域的技术。
结论：TB染色方法显示出作为识别组织和伤口中的发荧光和非发荧光生物膜的有效成像技术的巨大潜力。这种方法可以提高基于表面的治疗的精度和更好的患者结果。
目的：本研究的目的是开发一种依赖于死细胞的选择性锥虫蓝（TB）染色的成像技术，坏死组织，和细菌生物膜，识别组织和伤口上的生物膜区域。
方法：该研究探索了环境多色LED灯的组合，以在图像采集过程中获得染色的生物膜区域与下面的鸡组织或玻璃基材之间的最大差异。然后使用形状相似性测量将TB成像结果与荧光图像进行视觉和统计比较。
结果：拟议的TB染色方法与用于检测组织和玻璃基材上生物膜的荧光标准品之间的比较显示高达97％的相似性，表明TB染色方法是一种有前途的识别生物膜区域的技术。
结论：TB染色方法显示出作为识别组织和伤口中的发荧光和非发荧光生物膜的有效成像技术的巨大潜力。这种方法可以提高基于表面的治疗的精度和更好的患者结果。
