Abstract：
Few studies have investigated the long-term effect of exposure to arsenic (As), lead (Pb), and cadmium (Cd) via drinking water at the provisional guideline values on gut microflora. In this study, male and female mice were exposed to water As, Pb, or Cd at 10, 10, or 5 μg L-1 for 6 months. At the end of the exposure, the net weight gain of male mice exposed to As and Pb (9.91 ± 1.35 and 11.2 ± 1.50 g) was significantly (p < 0.05) lower compared to unexposed control mice (14.1 ± 3.24 g), while this was not observed for female mice. Relative abundance of Akkermansia, a protective gut bacterium against intestinal inflammation, was reduced from 29.7% to 3.20%, 4.83%, and 17.0% after As, Pb, and Cd exposure in male mice, which likely caused chronic intestinal inflammation, as suggested by 2.81- to 9.60-fold higher mRNA levels of pro-inflammatory factors in ileal enterocytes of male mice. These results indicate that long-term exposure to drinking water As, Pb, and Cd at concentrations equivalent to the China provisional guideline values can cause loss of protective bacteria and lead to chronic intestinal inflammation, thereby affecting body weight gain in male mice.
摘要：
很少有研究调查暴露于砷（As）的长期影响，铅(Pb),和镉（Cd）通过饮用水在肠道微生物区系的临时指导值。在这项研究中,雄性和雌性小鼠暴露于水中，Pb,或10、10或5μgL-1的Cd持续6个月。曝光结束时,与未暴露的对照小鼠（14.1±3.24g）相比，暴露于As和Pb的雄性小鼠的净重增长（9.91±1.35和11.2±1.50g）显着降低（p<0.05），而雌性小鼠没有观察到这一点。阿克曼西亚的相对丰度，一种针对肠道炎症的保护性肠道细菌，从29.7%降至3.20%，4.83%,和17.0%后，Pb,和雄性小鼠的镉暴露，这可能导致慢性肠道炎症，正如雄性小鼠回肠肠上皮细胞中促炎症因子mRNA水平高2.81至9.60倍所表明的那样。这些结果表明，长期接触饮用水As，Pb,和Cd在相当于中国临时指导值的浓度会导致保护性细菌的损失，并导致慢性肠道炎症，从而影响雄性小鼠的体重增加。
