Abstract：
The black soldier fly (BSF) rearing technology has been a promising bioconversion method for food waste (FW) disposal. However, when used independently, it currently only achieves low efficiency and biomass transformation rates (BTR). This study screened and identified two strains of gut beneficial bacteria, Bacillus cereus and Bacterium YC-LK-LKJ45. The efficiency of a complex culture formulated by these strains was investigated, focusing on enhancing FW reduction and high-value biomass production during the rearing of BSF larvae. The coculture agent group (G1-10%, with two strains in 1:1 volume ratio at a 10 % dosage) exhibited higher larval yield (627.67 g·kg-1), BTR (47.90 %), FW reduction efficiency (80.67 %), and total protein and fat yield (261.99 g·kg-1and 46.24 g·kg-1) compared to the control and the monoculture agent group (which added a single gut beneficial bacteria agent, either Bacillus cereus or Bacterium YC-LK-LKJ45). The bacterial agent altered the richness and diversity of the gut microbial community of BSF, increasing the relative abundance of beneficial bacteria such as Bacillus, Oceano bacillus, and Akkermansia, while decreasing pathogenic bacteria, such as Acinetobacter and Escherichia-Shigella. Structural equation model quantification revealed that α-diversity (λ = 0.897, p < 0.001) and BTR (λ = 0.747, p < 0.001) are crucial drivers for enhancing high-value biomass during bioaugmentation rearing. This investigation provides a theoretical framework for the effective management of food waste using BSF, enhancing its decomposition and transformation into higher-value biomass.
摘要：
黑兵蝇（BSF）饲养技术已成为一种有前途的食品废物（FW）处理生物转化方法。然而,当独立使用时，它目前仅实现低效率和生物量转化率（BTR）。本研究筛选并鉴定了两株肠道有益菌,蜡状芽孢杆菌和细菌YC-LK-LKJ45。研究了由这些菌株配制的复杂培养物的效率，重点是提高BSF幼虫饲养过程中的FW减少和高价值生物量生产。共培养剂组(G1-10%，以1：1的体积比在10％的剂量下使用两个菌株）表现出更高的幼虫产量（627.67g·kg-1），BTR（47.90%），FW降低效率（80.67%），和总蛋白质和脂肪产量（261.99g·kg-1和46.24g·kg-1）与对照组和单一培养剂（添加了单一肠道有益菌剂，蜡样芽孢杆菌或细菌YC-LK-LKJ45)。细菌剂改变了BSF肠道微生物群落的丰富度和多样性，增加有益细菌如芽孢杆菌的相对丰度，大洋杆菌,和Akkermansia,在减少致病菌的同时，例如不动杆菌和大肠杆菌-志贺氏菌。结构方程模型量化表明，α多样性（λ=0.897，p<0.001）和BTR（λ=0.747，p<0.001）是生物强化饲养过程中提高高价值生物量的关键驱动因素。这项调查为使用BSF有效管理食物垃圾提供了理论框架，增强其分解和转化为更高价值的生物质。
