Abstract：
The genus Amanita sect. Amanita harbors approximately 150 species in the world, and 27 species have been recognized in China. Some of the species in China have continuously caused poisoning. The responsible toxins should be ibotenic acid (IBO) and muscimol (MUS). However, species of the section Amanita containing IBO and MUS and their systematic positions are unclear. In this study, the contents of IBO and MUS in 84 samples of 24 species in section Amanita were detected using UPLC‒MS/MS, and the distribution of toxin-containing species in the molecular phylogeny was analyzed by the combined (ITS, nrLSU, RPB2, TUB2 and TEF1-α) dataset using maximum likelihood (ML) analysis and Bayesian inference (BI). Our results indicated that 10 of the 24 species contained IBO and MUS ranging from 0.6125 to 32.0932 and 0.0056-5.8685 g/kg dry weight, respectively. Among these 10 species, the toxins of eight species, including Amanita altipes, A. concentrica, A. flavopantherina, A. griseopantherina, A. pseudopantherina, A. rubrovolvata, A. subglobosa and A. sychnopyramis, were detected for the first time. In addition, the IBO and MUS contents of A. subglobosa in different growth stages showed that both toxins decreased in the mature stage. The phylogenetic analysis showed that all species of sect. Amanita from China were divided into 5 groups. And IBO- and MUS-containing species were gathered in clades Ⅰ and Ⅳ, but not all of the species in the two clades contain the toxins. No presence of IBO and MUS in the species of clades Ⅱ, Ⅲ and Ⅴ were confirmed.
摘要：
天牛属。世界上有大约150个物种，在中国已经确认了27种。中国的一些物种不断引起中毒。负责任的毒素应该是次品酸(IBO)和麝香酚(MUS)。然而,含有IBO和MUS的Amanita部分的物种及其系统位置尚不清楚。在这项研究中,采用UPLC-MS/MS检测了天牛24种84个样品中IBO和MUS的含量，并结合(ITS，nrLSU,RPB2、TUB2和TEF1-α)数据集使用最大似然(ML)分析和贝叶斯推断(BI)。我们的结果表明，24种物种中有10种含有IBO和MUS，范围为0.6125至32.0932和0.0056-5.8685g/kg干重，分别。在这10个物种中，八种毒素，包括AmanitaAltipes,A.精矿，A.flavopantherina，A.griseopantherina，A.假大麻，A.rubrovolvata，A.subglobosa和A.sychnopyramis，第一次被发现。此外,不同生长阶段球藻的IBO和MUS含量表明，两种毒素在成熟期均降低。系统发育分析表明，所有物种的教派。来自中国的Amanita分为5组。含IBO和MUS的物种聚集在进化枝Ⅰ和Ⅳ中，但并非这两个进化枝中的所有物种都含有毒素。在进化枝Ⅱ的物种中不存在IBO和MUS，Ⅲ和Ⅴ得到证实。
