PDF(Pubmed)
Abstract：
A structural protein called keratin is often employed in the medical industry to create medication carriers. Process improvement, antioxidant, antibacterial, and adjuvant drug studies of synthetic bioactive keratin microparticles made from lipids and keratin derived from porcupine (Hystrix indica) quills are the main objectives of this study. After coating the keratin microparticles with lipids which were obtained from the same porcupine quills, the bioactive keratin microparticles were produced. The response surface technique was applied to optimize the conditions for extraction of the keratin protein and sizing of the keratin microparticles. An infrared spectroscopy was used to analyze the chemical shifts in compositions of keratin microparticles while the optical microscopy was used to measure the size of the keratin microparticles. The results of this work revealed that a yield 27.36 to 42.25% of the keratin protein could be obtained from porcupine quills. The keratin microparticles were sized between 60.65 and 118.87 µm. Through response surface optimization, mercaptoethanol and urea were shown to be the main variables which positively affected the yield and the size of the keratin protein. The lipid stacking on the keratin microparticles\' surface was confirmed by infrared spectroscopy. The 2,2\'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) assay confirmed the keratin microparticle\'s antioxidant activity of 29.83%. Compared to lipid alone, the antibacterial properties of the keratin microparticles against Escherichia coli-a gram-negative-and Staphylococcus aureus-a gram-positive-bacteria enhanced by up to 55% following the coating of the microparticles with the lipids. The pharmacological action against these bacterial species was further improved by the lipid-loaded erythromycin that was carried on the surface of keratin microparticles. This work has demonstrated the design and uses of the keratin microparticles obtained from porcupine quills for clinical applications.
摘要：
称为角蛋白的结构蛋白通常用于医疗行业以创建药物载体。流程改进,抗氧化剂，抗菌，和辅助药物研究的合成生物活性角蛋白微粒由脂质和源自豪猪（Hystrixindica）羽毛的角蛋白制成，是本研究的主要目标。用从相同豪猪毛笔获得的脂质涂覆角蛋白微粒后，产生了生物活性角蛋白微粒。应用响应面技术优化了角蛋白提取和角蛋白微粒大小的条件。使用红外光谱法分析角蛋白微粒的组成中的化学位移,同时使用光学显微镜测量角蛋白微粒的尺寸。这项工作的结果表明，从豪猪刺中可以获得27.36至42.25％的角蛋白。角蛋白微粒的大小在60.65和118.87μm之间。通过响应面优化,巯基乙醇和尿素被证明是对角蛋白的产量和大小产生积极影响的主要变量。通过红外光谱法证实了角蛋白微粒表面上的脂质堆积。2,2'-嗪双-(3-乙基苯并噻唑啉-6-磺酸盐)测定证实角蛋白微粒的抗氧化活性为29.83%。与单独的脂质相比，在用脂质涂覆微粒后，角蛋白微粒对大肠杆菌-革兰氏阴性-和金黄色葡萄球菌-革兰氏阳性-细菌的抗菌性能增强了高达55％。通过在角蛋白微粒表面上携带的负载脂质的红霉素，对这些细菌物种的药理作用得到了进一步改善。这项工作已经证明了从豪猪毛笔获得的用于临床应用的角蛋白微粒的设计和用途。
