Spontaneous activity of neuronal cultures from embryonic cortices was recorded using 60-electrode multi-electrode arrays (MEAs) between 17 and 27 days in vitro. The neuronal cultures were subjected to 15 min RF exposures at SAR of 1, 3, and 28 W/kg.
At SAR close to the guidelines (1 and 3 W/kg), we found no conclusive evidence that 3.5 GHz RF exposure impacts the activity of neurons in vitro. On the contrary, CW and 5G-modulated signals elicited a clear decrease in bursting and total firing rates during RF exposure at high SAR levels (28 W/kg). Our experimental findings extend our previous results, showing that RF, at 1.8 to 3.5 GHz, inhibits the electrical activity of neurons in vitro at levels above environmental standards.
■使用60电极多电极阵列(MEAs)在体外17至27天之间记录了来自胚胎皮质的神经元培养物的自发活动。对神经元培养物进行15分钟的RF暴露,SAR为1、3和28W/kg。
■在SAR接近准则(1和3W/kg)时,我们发现没有确凿的证据表明3.5GHz射频暴露会影响体外神经元的活动.相反,在高SAR水平(28W/kg)下,CW和5G调制信号在RF暴露期间引起的爆发率和总激发率明显下降。我们的实验发现扩展了我们之前的结果,显示RF,在1.8到3.5GHz,在高于环境标准的水平上抑制体外神经元的电活动。