PDF(Pubmed)
Abstract：
The current deployment of the fifth generation (5G) of wireless communications raises new questions about the potential health effects of exposure to radiofrequency (RF) fields. So far, most of the established biological effects of RF have been known to be caused by heating. We previously reported inhibition of the spontaneous electrical activity of neuronal networks in vitro when exposed to 1.8 GHz signals at specific absorption rates (SAR) well above the guidelines. The present study aimed to assess the effects of RF fields at 3.5 GHz, one of the frequencies related to 5G, on neuronal activity in-vitro. Potential differences in the effects elicited by continuous-wave (CW) and 5G-modulated signals were also investigated.
Spontaneous activity of neuronal cultures from embryonic cortices was recorded using 60-electrode multi-electrode arrays (MEAs) between 17 and 27 days in vitro. The neuronal cultures were subjected to 15 min RF exposures at SAR of 1, 3, and 28 W/kg.
At SAR close to the guidelines (1 and 3 W/kg), we found no conclusive evidence that 3.5 GHz RF exposure impacts the activity of neurons in vitro. On the contrary, CW and 5G-modulated signals elicited a clear decrease in bursting and total firing rates during RF exposure at high SAR levels (28 W/kg). Our experimental findings extend our previous results, showing that RF, at 1.8 to 3.5 GHz, inhibits the electrical activity of neurons in vitro at levels above environmental standards.
Spontaneous activity of neuronal cultures from embryonic cortices was recorded using 60-electrode multi-electrode arrays (MEAs) between 17 and 27 days in vitro. The neuronal cultures were subjected to 15 min RF exposures at SAR of 1, 3, and 28 W/kg.
At SAR close to the guidelines (1 and 3 W/kg), we found no conclusive evidence that 3.5 GHz RF exposure impacts the activity of neurons in vitro. On the contrary, CW and 5G-modulated signals elicited a clear decrease in bursting and total firing rates during RF exposure at high SAR levels (28 W/kg). Our experimental findings extend our previous results, showing that RF, at 1.8 to 3.5 GHz, inhibits the electrical activity of neurons in vitro at levels above environmental standards.
摘要：
■当前第五代（5G）无线通信的部署提出了有关暴露于射频（RF）场的潜在健康影响的新问题。到目前为止,已知RF的大多数已建立的生物效应是由加热引起的。我们先前报道了以远高于指南的比吸收率（SAR）暴露于1.8GHz信号时，体外神经网络的自发电活动受到抑制。本研究旨在评估3.5GHz射频场的影响，与5G相关的频率之一，体外神经元活性。还研究了连续波（CW）和5G调制信号引起的效应的潜在差异。
■使用60电极多电极阵列（MEAs）在体外17至27天之间记录了来自胚胎皮质的神经元培养物的自发活动。对神经元培养物进行15分钟的RF暴露，SAR为1、3和28W/kg。
■在SAR接近准则（1和3W/kg）时，我们发现没有确凿的证据表明3.5GHz射频暴露会影响体外神经元的活动.相反,在高SAR水平（28W/kg）下，CW和5G调制信号在RF暴露期间引起的爆发率和总激发率明显下降。我们的实验发现扩展了我们之前的结果，显示RF，在1.8到3.5GHz，在高于环境标准的水平上抑制体外神经元的电活动。
■使用60电极多电极阵列（MEAs）在体外17至27天之间记录了来自胚胎皮质的神经元培养物的自发活动。对神经元培养物进行15分钟的RF暴露，SAR为1、3和28W/kg。
■在SAR接近准则（1和3W/kg）时，我们发现没有确凿的证据表明3.5GHz射频暴露会影响体外神经元的活动.相反,在高SAR水平（28W/kg）下，CW和5G调制信号在RF暴露期间引起的爆发率和总激发率明显下降。我们的实验发现扩展了我们之前的结果，显示RF，在1.8到3.5GHz，在高于环境标准的水平上抑制体外神经元的电活动。
