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Abstract：
Viral inclusion bodies (IBs) are potential sites of viral replication and assembly. How viral IBs form remains poorly defined. Here we describe a combined biophysical and cellular approach to identify the components necessary for IB formation during Ebola virus (EBOV) infection. We find that the eNP0VP35 complex containing Ebola nucleoprotein (eNP) and viral protein 35 (eVP35), the functional equivalents of nucleoprotein (N) and phosphoprotein (P) in non-segmented negative strand viruses (NNSVs), phase separates to form inclusion bodies. Phase separation of eNP0VP35 is reversible and modulated by ionic strength. The multivalency of eVP35, and not eNP, is also critical for phase separation. Furthermore, overexpression of an eVP35 peptide disrupts eNP0VP35 complex formation, leading to reduced frequency of IB formation and limited viral infection. Together, our results show that upon EBOV infection, the eNP0VP35 complex forms the minimum unit to drive IB formation and viral replication.
摘要：
病毒包涵体(IBs)是病毒复制和组装的潜在位点。病毒IBs如何形成仍然不明确。在这里，我们描述了一种结合的生物物理和细胞方法，以鉴定埃博拉病毒（EBOV）感染期间IB形成所需的成分。我们发现，eNP0VP35复合物含有埃博拉病毒核蛋白（eNP）和病毒蛋白35（eVP35），非分段负链病毒（NSSV）中核蛋白（N）和磷蛋白（P）的功能等价物，相分离形成包涵体。eNP0VP35的相分离是可逆的并且受离子强度调节。eVP35的多价，而不是eNP，对于相分离也是至关重要的。此外，eVP35肽的过表达破坏eNP0VP35复合物的形成，导致IB形成频率降低和有限的病毒感染。一起,我们的结果表明，在EBOV感染后，eNP0VP35复合物形成驱动IB形成和病毒复制的最小单位。
