Abstract：
Nimetazepam (marketed brand names; Erimin and Lavol) is an intermediate acting benzodiazepine derivative, which was widely used mainly in East and Southeast Asian region countries including Japan, Malaysia, Brunei, the Philippines, Thailand, Indonesia, Hong Kong, Singapore and China. Nimetazepam and its metabolite 7-aminonimetazepam were quantified from human hair samples by liquid chromatography tandem-mass spectrometry (LC-MS/MS), under selective reaction monitoring mode. Using diazepam-d5 as an internal standard, the concentration of nimetazepam and its metabolite 7-aminonimetazepam could be determined by matrix matched calibration method. Extraction of the target compounds was performed by using methanol, followed by evaporation and being concentrated with nitrogen. The Limit of quantification concentrations of nimetazepam and its metabolite 7-aminonimetazepam in hair samples were both 25 pg/mg by established method. The concentrations of nimetazepam in hair samples obtained from 2 users were 27.4, and 22.0 pg/mg, respectively; the concentrations of 7-animonimetazepam in hair samples were 54.2 and 29.1 pg/mg, respectively. In our study, the 7-aminonimetazepam concentrations in hair was higher than those of nimetazepam in the authentic hair samples. To our knowledge, this is the first report to establish the detailed procedure for quantificating nimetazepam and 7-aminonimetazepam in human hair by LC-MS/MS.
摘要：
Nimetazepam（市售品牌名称；Erimin和Lavol）是一种中效苯二氮卓衍生物，主要在包括日本在内的东亚和东南亚地区国家广泛使用，马来西亚,文莱,菲律宾,泰国,印度尼西亚,香港,新加坡和中国。通过液相色谱串联质谱法（LC-MS/MS）从人发样品中定量尼美西m及其代谢物7-氨基尼美西m，在选择性反应监测模式下。使用地西泮-d5作为内标，尼美西泮及其代谢物7-氨基西泮的浓度可通过基质匹配校准法测定。目标化合物的提取通过使用甲醇进行,然后蒸发并用氮气浓缩。根据已建立的方法，头发样品中尼美西泮及其代谢产物7-氨基西泮的定量浓度极限均为25pg/mg。从2名使用者获得的头发样本中尼美西泮的浓度分别为27.4和22.0pg/mg，头发样本中7-亚依米美西泮的浓度分别为54.2和29.1pg/mg，分别。在我们的研究中,在真实的头发样本中，头发中的7-氨基西泮浓度高于尼美西泮。据我们所知,这是建立通过LC-MS/MS定量人发中尼美西泮和7-氨基西泮的详细程序的第一份报告。
