Abstract：
Visual detection assay based on aptamer unmodified gold nanoparticles shows great potential in biotechnology. Here, we reported a visible, salt-free and highly sensitive tetracycline (TC) assay based on a colloidally stable mixture of AuNPs that contains poly (diallyl dimethylammonium chloride) capped AuNPs ((+)AuNPs) and tetracycline-specific aptamer capped AuNPs (Apt-capped AuNPs). This reported TC assay was visible and salt-free that did not need any salt during TC detection. With naked eyes, nanomolar tetracycline concentrations could be identified within 20 min. A detection limit of tetracycline down to a concentration of 1.0 fM with a broad detection range of 8 order of magnitudes (5 × 10-14 M to 5 × 10-6 M) was reached. Furthermore, the reported TC assay also exhibited good selectivity for tetracycline over other antibiotics, metal cations, proteins and amino acids. These findings clearly demonstrated the high potential of the reported TC assay for TC detection and monitoring.
摘要：
基于适体未修饰的金纳米颗粒的视觉检测实验在生物技术中显示出巨大的潜力。这里,我们报告了一个可见的，无盐和高度敏感的四环素（TC）测定基于胶态稳定的AuNP混合物，该混合物包含聚（二烯丙基二甲基氯化铵）封端的AuNP（（（））AuNP）和四环素特异性适体封端的AuNP（Apt封端的AuNP）。该报告的TC测定是可见的且无盐的,其在TC检测期间不需要任何盐。用裸眼,纳摩尔四环素浓度可以在20分钟内确定。四环素的检测极限低至1.0fM的浓度，检测范围为8个数量级（5×10-14M至5×10-6M）。此外，报道的TC测定对四环素的选择性也优于其他抗生素，金属阳离子，蛋白质和氨基酸。这些发现清楚地证明了所报道的TC测定用于TC检测和监测的高潜力。
