PDF(Pubmed)
Abstract：
Canastra Minas Artisanal Cheese is produced in the Brazilian State of Minas Gerais using raw milk, rennet, and pingo, a natural endogenous starter culture (fermented whey) collected from the previous day\'s production. Due to the use of raw milk, the product can carry microorganisms that may cause foodborne diseases (FBD), including Staphylococcus aureus. Genomic characterization of S. aureus is an important tool to assess diversity, virulence, antimicrobial resistance, and the potential for causing food poisoning due to enterotoxin production. This study is aimed at exploring the genomic features of S. aureus strains isolated from Canastra Minas Artisanal Cheeses. Multilocus sequence typing (MLST) classified these strains as ST1, ST5, and a new profile ST7849 (assigned to the clonal complex CC97). These strains belonged to four spa types: t008, t127, t359, and t992. We identified antimicrobial resistance genes with phenotypic correlation against methicillin (MRSA) and tetracycline. Virulome analysis revealed genes associated with iron uptake, immune evasion, and potential capacity for adherence and biofilm formation. The toxigenic potential included cyto- and exotoxins genes, and all strains presented the genes that encode for Panton-Valentine toxin and hemolysin, and two strains encoded 4 and 8 Staphylococcal enterotoxin (SE) genes. The results revealed the pathogenic potential of the evaluated S. aureus strains circulating in the Canastra region, representing a potential risk to public health. This study also provides useful information to monitor and guide the application of control measures to the artisanal dairy food production chain.
摘要：
CanastraMinas手工奶酪是在巴西米纳斯吉拉斯州使用原料奶生产的，rennet,还有Pingo,从前一天的生产中收集的天然内源发酵剂（发酵乳清）。由于使用原料奶，该产品可以携带可能导致食源性疾病（FBD）的微生物，包括金黄色葡萄球菌.金黄色葡萄球菌的基因组表征是评估多样性的重要工具，毒力,抗菌素耐药性，以及由于肠毒素的产生而导致食物中毒的可能性。本研究旨在探索从CanastraMinas手工奶酪中分离出的金黄色葡萄球菌菌株的基因组特征。多位点序列分型（MLST）将这些菌株分类为ST1，ST5和新的图谱ST7849（分配给克隆复合物CC97）。这些菌株属于四种spa类型:t008、t127、t359和t992。我们确定了与甲氧西林（MRSA）和四环素表型相关的抗菌素抗性基因。病毒组分析显示与铁摄取相关的基因，免疫逃避,以及粘附和生物膜形成的潜在能力。产毒潜力包括细胞和外毒素基因，所有菌株都提供了编码潘顿-瓦伦丁毒素和溶血素的基因，两个菌株编码4和8个葡萄球菌肠毒素（SE）基因。结果揭示了在Canastra地区循环的评估的金黄色葡萄球菌菌株的致病潜力，对公众健康构成潜在风险。这项研究还提供了有用的信息，以监测和指导控制措施在手工乳制品生产链中的应用。
