Abstract：
Gastrodia elata needs to establish a symbiotic relationship with Armillaria strains to obtain nutrients and energy. However, the signaling cross talk between G. elata and Armillaria strains is still unclear. During our experiment, we found that the vegetative mycelium of Armillaria gallica 012m grew significantly better in the media containing gibberellic acid (GA3) than the blank control group (BK). To explore the response mechanism, we performed an RNA-sequencing experiment to profile the transcriptome changes of A. gallica 012m cultured in the medium with exogenous GA3. The transcriptome-guided differential expression genes (DEGs) analysis of GA3 and BK showed that a total of 1309 genes were differentially expressed, including 361 upregulated genes and 948 downregulated genes. Some of those DEGs correlated with the biological process, including positive regulation of chromosome segregation, mitotic metaphase/anaphase transition, attachment of mitotic spindle microtubules to kinetochore, mitotic cytokinesis, and nuclear division. These analyses explained that GA3 actively promoted the growth of A. gallica to some extent. Further analysis of protein domain features showed that the deduced polypeptide contained 41 candidate genes of GA receptor, and 27 of them were expressed in our samples. We speculate that GA receptors exist in A. gallica 012m. Comparative studies of proteins showed that the postulated GA receptor domains of A. gallica 012m have a higher homologous correlation with fungi than others based on cluster analysis.
摘要：
天麻需要与蜜环菌菌株建立共生关系以获得营养和能量。然而,G.elata和蜜环菌菌株之间的信号串扰仍不清楚。在我们的实验中,我们发现，蜜环菌012m的营养菌丝体在含赤霉素（GA3）的培养基中的生长明显优于空白对照组（BK）。探索反应机制，我们进行了RNA测序实验，以描述在外源GA3培养基中培养的A.gallica012m的转录组变化。GA3和BK的转录组指导差异表达基因(DEGs)分析表明,共有1309个基因差异表达,包括361个上调基因和948个下调基因。其中一些DEGs与生物过程相关，包括染色体分离的正调节，有丝分裂中期/后期过渡，有丝分裂纺锤体微管与动粒的附着，有丝分裂胞质分裂,核分裂。这些分析解释了GA3在一定程度上积极促进了紫菜的生长。进一步的蛋白质结构域特征分析表明，推导的多肽含有41个GA受体候选基因，其中27个在我们的样本中表达。我们推测GA受体存在于五倍子012m中。蛋白质的比较研究表明，基于聚类分析，Gallica012m的假定GA受体结构域与真菌的同源性较高。
