Abstract：
The green production of silver nanoparticles (AgNPs) produces AgNPs with minimum influence on the environment by using plant components such as alkaloids, carbohydrates, lipids, enzymes, flavonoids, terpenoids, and polyphenols as reducing agents. In the present investigation, Azadirachta indica leaf extract was used to form AgNPs from a 1 mM silver nitrate solution. The plan proved to be incredibly straightforward, cost-effective, and effective. The production of the nanoparticles was observed visually, where the colorless fluid turns into a brown-colored solution. Further research was carried out using x-ray diffraction, Fourier-transform infrared analysis, scanning electron microscopy, and transmission electron microscopy (TEM) in addition to UV-visible spectroscopy. The size range of AgNPs determined by TEM was 10-30 nm. When the diffusion technique was employed to demonstrate the antibacterial effect of AgNPs on various pathogens, the zones of inhibition for Staphylococcus aureus, Bacillus cereus, and Escherichia coli, when 50 g of AgNPs were used were 16, 12, and 17 mm, respectively. By examining the leakage of reducing sugars and proteins, the mechanism by which nanoparticle antibacterial properties were explored, showed that AgNPs were capable of lowering membrane permeability.
摘要：
银纳米粒子（AgNPs）的绿色生产通过使用生物碱等植物成分来产生对环境的影响最小的AgNPs，碳水化合物,脂质，酶，黄酮类化合物，萜类化合物,和多酚作为还原剂。在目前的调查中,用印染氮芥叶提取物从1mM硝酸银溶液中形成AgNP。这个计划被证明是非常简单的，成本效益高,而且有效。目视观察纳米颗粒的产生，无色液体变成棕色溶液。使用X射线衍射进行了进一步的研究，傅里叶变换红外分析,扫描电子显微镜，和透射电子显微镜(TEM)以及紫外可见光谱。通过TEM确定的AgNP的尺寸范围为10-30nm。当使用扩散技术来证明AgNPs对各种病原体的抗菌作用时，金黄色葡萄球菌的抑制区，蜡样芽孢杆菌,和大肠杆菌，当使用50克AgNPs时，它们分别为16、12和17毫米，分别。通过检查还原糖和蛋白质的泄漏，探索纳米粒子抗菌性能的机理，表明AgNPs能够降低膜的通透性。
