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Abstract：
Calcium imaging is commonly used to visualize neural activity in vivo. In particular, mesoscale calcium imaging provides large fields of view, allowing for the simultaneous interrogation of neuron ensembles across the neuraxis. In the field of Developmental Neuroscience, mesoscopic imaging has recently yielded intriguing results that have shed new light on the ontogenesis of neural circuits from the first stages of life. We summarize here the technical approaches, basic notions for data analysis and the main findings provided by this technique in the last few years, with a focus on brain development in mouse models. As new tools develop to optimize calcium imaging in vivo, basic principles of neural development should be revised from a mesoscale perspective, that is, taking into account widespread activation of neuronal ensembles across the brain. In the future, combining mesoscale imaging of the dorsal surface of the brain with imaging of deep structures would ensure a more complete understanding of the construction of circuits. Moreover, the combination of mesoscale calcium imaging with other tools, like electrophysiology or high-resolution microscopy, will make up for the spatial and temporal limitations of this technique.
摘要：
钙成像通常用于观察体内的神经活动。特别是,中尺度钙成像提供了大视场，允许同时询问跨神经轴的神经元集合。在发展神经科学领域，介观成像最近产生了有趣的结果，这些结果为从生命的最初阶段开始的神经回路的本体发生提供了新的启示。我们在这里总结了技术方法，数据分析的基本概念以及该技术在过去几年中提供的主要发现，专注于小鼠模型中的大脑发育。随着优化体内钙成像的新工具的发展，神经发育的基本原理应该从中尺度的角度进行修正，也就是说,考虑到整个大脑中神经元集合的广泛激活。在未来,将大脑背侧表面的中尺度成像与深层结构的成像相结合，将确保对电路结构的更完整理解。此外，中尺度钙成像与其他工具的结合，如电生理学或高分辨率显微镜，将弥补这种技术的空间和时间限制。
