PDF(Pubmed)
Abstract：
This study aimed to investigate whether α-fetoprotein (AFP) could affect the malignant behavior of AFP-producing gastric cancer (AFP-GC) and to explore the relationship between AFP and mesenchymal-epithelial transition factor (c-Met) in AFP-GC. In this study, 23 patients with AFP-GC (AFP[+]) and 18 patients with common gastric cancer (AFP[-]) were evaluated for the c-Met expression using immunohistochemical analysis. The AFP-GC cell line, GCIY, was used. The AFP endoribonuclease-prepared small interfering RNA (siRNA) and eukaryotic AFP overexpression vector were used to increase/knockdown the expression of AFP. Afterward, the c-Met expression was evaluated by polymerase chain reaction and western blot. The proliferation, migration, and invasion of GCIY cells were estimated before and after the AFP overexpression/knockdown. The c-Met expression in both groups was the same (p > 0.05), and AFP[+] group had a higher positive incidence of the c-Met expression than the AFP[-] group (p < 0.01). Furthermore, the c-Met expression frequency was decreased by AFP knockdown and increased by AFP overexpression (p < 0.01). The cell counting kit-8 cell proliferation assay, cell invasion, and migration assays confirmed that the AFP could affect the malignant biological behavior of AFP-GC. These findings suggest that AFP contributes to the malignant biological properties of AFP-GC and the high expression of c-Met in AFP-GC.
摘要：
本研究旨在探讨甲胎蛋白(AFP)是否会影响产AFP胃癌(AFP-GC)的恶性行为，并探讨AFP与间充质上皮转化因子(c-Met)在AFP-GC中的关系。在这项研究中,使用免疫组织化学分析评估了23例AFP-GC患者（AFP[]）和18例普通胃癌患者（AFP[-]）的c-Met表达。AFP-GC细胞系，GCIY,被使用。AFP核糖核酸内切酶制备的小干扰RNA（siRNA）和真核AFP过表达载体用于增加/敲低AFP的表达。之后,通过聚合酶链反应和蛋白质印迹评估c-Met表达。扩散,迁移,在AFP过表达/敲低之前和之后估计GCIY细胞的侵袭。两组的c-Met表达水平相同(p>0.05),AFP[+]组c-Met表达阳性率高于AFP[-]组(p<0.01)。此外，c-Met表达频率因AFP敲低而降低，因AFP过表达而增加（p<0.01）。细胞计数试剂盒-8细胞增殖试验，细胞入侵，和迁移实验证实AFP可以影响AFP-GC的恶性生物学行为。这些发现表明AFP有助于AFP-GC的恶性生物学特性和c-Met在AFP-GC中的高表达。
