Abstract：
In the Czech Republic, botulism is a rare life-threatening disease. A total of 155 cases have been reported since 1960; according to the ISIN (formerly EPIDAT) database, there have been only three isolated cases since 2013, with the exception of a single occurrence of familial botulism in 2013. In our work, we present the occurrence of botulism after ingestion of pâté of untraceable origin by a couple who were hospitalized for botulotoxin food poisoning in July 2022. Their neurological symptoms were dominated by dysarthria. After administration of antibotulinum serum, their condition improved significantly. Patient samples were analyzed using affinity carriers and MALDI mass spectrometry, a modern highly sensitive technique for detecting the presence of botulinum neurotoxins. Unlike traditional detection by a difficult and costly biological experiment on mice, the above analysis does not require the killing of laboratory animals.
摘要：
在捷克共和国,肉毒中毒是一种罕见的危及生命的疾病。自1960年以来，共报告了155例病例；根据ISIN（以前的EPIDAT）数据库，自2013年以来，只有3例孤立病例，但2013年仅有一例家族性肉毒中毒。在我们的工作中,我们介绍了在2022年7月因肉毒毒素食物中毒住院的一对夫妇摄入了无法追踪的来源的pté后发生肉毒杆菌中毒的情况.他们的神经症状主要是构音障碍。服用抗肉毒杆菌血清后，他们的状况明显改善。使用亲和载体和MALDI质谱分析患者样品，检测肉毒神经毒素存在的现代高灵敏度技术。与传统的通过对小鼠进行困难且昂贵的生物实验进行检测不同，上述分析不需要杀死实验动物。
