Abstract：
To explore the differentially expressed genes (DEGs) related to biosynthesis of active ingredients in wolfberry fruits of different varieties of Lycium barbarum L. and reveal the molecular mechanism of the differences of active ingredients, we utilized Illumina NovaSeq 6000 high-throughput sequencing technology to conduct transcriptome sequencing on the fruits of \'Ningqi No.1\' and \'Ningqi No.7\' during the green fruit stage, color turning stage and maturity stage. Subsequently, we compared the profiles of related gene expression in the fruits of the two varieties at different development stages. The results showed that a total of 811 818 178 clean reads were obtained, resulting in 121.76 Gb of valid data. There were 2 827, 2 552 and 2 311 DEGs obtained during the green fruit stage, color turning stage and maturity stage of \'Ningqi No. 1\' and \'Ningqi No. 7\', respectively, among which 2 153, 2 050 and 1 825 genes were annotated in six databases, including gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG) and clusters of orthologous groups of proteins (KOG). In GO database, 1 307, 865 and 624 DEGs of green fruit stage, color turning stage and maturity stage were found to be enriched in biological processes, cell components and molecular functions, respectively. In the KEGG database, the DEGs at three developmental stages were mainly concentrated in metabolic pathways, biosynthesis of secondary metabolites and plant-pathogen interaction. In KOG database, 1 775, 1 751 and 1 541 DEGs were annotated at three developmental stages, respectively. Searching the annotated genes against the PubMed database revealed 18, 26 and 24 DEGs related to the synthesis of active ingredients were mined at the green fruit stage, color turning stage and maturity stage, respectively. These genes are involved in carotenoid, flavonoid, terpenoid, alkaloid, vitamin metabolic pathways, etc. Seven DEGs were verified by RT-qPCR, which showed consistent results with transcriptome sequencing. This study provides preliminary evidences for the differences in the content of active ingredients in different Lycium barbarum L. varieties from the transcriptional level. These evidences may facilitate further exploring the key genes for active ingredients biosynthesis in Lycium barbarum L. and analyzing their expression regulation mechanism.
摘要：
探讨不同品种枸杞果实中活性成分生物合成的差异表达基因(DEGs),揭示活性成分差异的分子机制。我们利用IlluminaNovaSeq6000高通量测序技术对绿色果实阶段的“宁琪一号”和“宁琪七号”果实进行了转录组测序，颜色转向阶段和成熟阶段。随后，我们比较了两个品种不同发育阶段果实中相关基因的表达谱。结果表明，总共获得了811818178个干净的读数，产生121.76Gb的有效数据。在绿色果实阶段获得了2827、2552和2311DEGs，宁启号的颜色转折阶段和成熟阶段。1\'和\'宁启编号。7\'，分别,其中在六个数据库中注释了2153、2050和1825个基因，包括基因本体论(GO)，京都基因和基因组百科全书（KEGG）和直系同源蛋白质群（KOG）。在GO数据库中，绿果期1307、865和624DEGs，颜色转向阶段和成熟阶段被发现在生物过程中富集，细胞成分和分子功能，分别。在KEGG数据库中，三个发育阶段的DEGs主要集中在代谢途径中，次生代谢产物的生物合成和植物-病原体相互作用。在KOG数据库中，在三个发育阶段注释了1775、1751和1541个DEGs，分别。针对PubMed数据库搜索注释的基因显示，在绿色果实阶段开采了与活性成分合成相关的18、26和24个DEGs，颜色转向阶段和成熟阶段，分别。这些基因与类胡萝卜素有关，类黄酮，萜类,生物碱,维生素代谢途径，等。通过RT-qPCR验证了七个DEGs，与转录组测序结果一致。本研究从转录水平为不同品种枸杞中有效成分含量的差异提供了初步证据。这些证据可能有助于进一步探索枸杞活性成分生物合成的关键基因并分析其表达调控机制。
