Abstract：
In addition to being the first NLR protein proposed to form inflammasome, NLRP1s have attracted much attention in their activation mechanism by post-translational auto-proteolysis to generate C-terminal CARD containing fragment to form inflammasome. Among NLRP1, mouse NLRP1B but not human NLRP1 is well studied for its activation by lethal toxin. As dissecting the cellular components involved in NLRP1-associated diseases is highly dependent on NLRP1 inflammasome activation, experiments that can lead to NLRP1 activation is of pivotal importance to elucidate the biological role and the activation mechanism of NLRP1 especially in human. In this chapter we describe methods commonly used for mouse NLRP1B inflammasome activation as well as activation of human NLRP1 inflammasome visualized by ASC speck formation in our laboratory.
摘要：
除了是第一个被提议形成炎性体的NLR蛋白，NLRP1通过翻译后自身蛋白水解产生含有C末端CARD的片段形成炎性体，其激活机制备受关注。在NLRP1中，对小鼠NLRP1B而不是人NLRP1的致死毒素激活进行了充分研究。由于解剖参与NLRP1相关疾病的细胞成分高度依赖于NLRP1炎性体激活,可以导致NLRP1激活的实验对于阐明NLRP1的生物学作用和激活机制至关重要，尤其是在人类中。在本章中，我们描述了通常用于小鼠NLRP1B炎性体激活以及在我们实验室中通过ASC斑点形成可视化的人NLRP1炎性体激活的方法。
