UNASSIGNED: A conjugated ultrashort antimicrobial peptide (KW-23) was designed and synthesized. KW-23 was challenged against standard and multidrug-resistant C. albicans alone and in combination with fluconazole using standard antimicrobial and checkerboard assays. The toxicity of the peptide was examined using hemolytic assays.
UNASSIGNED: KW-23 positively affected the standard and resistant Candidal strains (at 5 and 15 μg/mL respectively), exhibiting potent synergistic antimicrobial activity against the standard strain when combined with fluconazole. The effect of the combination was additive against the resistant strain (0.6 μg/mL). Furthermore, the peptide exhibited negligible toxicity on human erythrocytes.
UNASSIGNED: KW-23 and its combination with fluconazole could be a promising candidate for developing anticandidal agents.
■设计并合成了一种共轭超短抗菌肽(KW-23)。使用标准抗微生物和棋盘测定,KW-23单独和与氟康唑组合对标准和多重耐药性白色念珠菌进行了挑战。使用溶血测定法检查肽的毒性。
■KW-23对标准和抗性念珠菌菌株(分别为5和15μg/mL)产生积极影响,当与氟康唑联合使用时,对标准菌株表现出有效的协同抗菌活性。该组合对抗性菌株(0.6μg/mL)的作用是累加的。此外,该肽对人红细胞的毒性可忽略不计。
■KW-23及其与氟康唑的组合可能是开发抗虫药的有希望的候选药物。