PDF(Pubmed)
Abstract：
Construction of artificial cells requires the development of straightforward methods for mimicking natural phospholipid membrane formation. Here we describe the use of direct aminolysis ligations to spontaneously generate biomimetic phospholipid membranes from water-soluble starting materials. Additionally, we explore the suitability of such biomimetic approaches for driving the in situ formation of native phospholipid membranes. Our studies suggest that non-enzymatic ligation reactions could have been important for the synthesis of phospholipid-like membranes during the origin of life, and might be harnessed as simplified methods to enable the generation of lipid compartments in artificial cells.
摘要：
人造细胞的构建需要开发模拟天然磷脂膜形成的直接方法。在这里，我们描述了使用直接氨解连接从水溶性起始材料自发产生仿生磷脂膜。此外,我们探讨了这种仿生方法是否适合驱动天然磷脂膜的原位形成。我们的研究表明，在生命起源期间，非酶连接反应可能对磷脂样膜的合成很重要。并可能被用作简化的方法，以在人造细胞中产生脂质隔室。
