UNASSIGNED: Blood samples (n = 536) were collected from clinically symptomatic and asymptomatic domesticated animals and subjected to Giemsa staining and examined microscopically for the identification of Theileria.
UNASSIGNED: Smears showed an overall rate of positivity for Theileria spp. in 325/536 (60.6%) animals. Different infection rates were recorded across the various animal groups in the different study areas (Middle region 215/386 [55.7%], East region 100/139 [71.9%]). Of the 11 goat samples collected from the North region, 10 (90%) were positive. Infection rates per animal group based on microscopy were as follows: camels, 3/35 (8.5%); cows, 19/36 (52.7%); goats, 200/303 (66%); and sheep, 103/162 (63.5%). Real-time polymerase chain reaction confirmation of all microscopy-positive samples identified 23/325 (7.1%) results as false-positive.
UNASSIGNED: This study clarified that Theileria spp. is present in the Middle (Sharjah, Umm Al Quwain, and Ajman), East, and North regions. This report also confirmed the use of direct microscopy with Giemsa-stained blood films as the method of choice for diagnosing acute infections. Further work is needed to molecularly determine the prevalence and species of Theileria spp. circulating in the different parts of the UAE.
■从临床上有症状和无症状的驯养动物中收集血样(n=536),并进行Giemsa染色,并用显微镜检查以鉴定Theileria。
■涂片显示对Theileriaspp的总体阳性率。在325/536(60.6%)动物中。在不同研究区域的不同动物组中记录了不同的感染率(中部地区215/386[55.7%],东部地区100/139[71.9%])。在从北部地区收集的11只山羊样本中,10(90%)呈阳性。基于显微镜的每个动物组的感染率如下:骆驼,3/35(8.5%);奶牛,19/36(52.7%);山羊,200/303(66%);和绵羊,103/162(63.5%)。所有显微镜阳性样品的实时聚合酶链反应确认23/325(7.1%)结果为假阳性。
■这项研究澄清了Theileriaspp。存在于中部(沙迦,嗯AlQuwain,和阿治曼),东,和北部地区。该报告还证实了使用Giemsa染色的血膜直接显微镜作为诊断急性感染的首选方法。需要进一步的工作来从分子上确定Theileriaspp的患病率和种类。在阿联酋的不同地区流通。