PDF(Pubmed)
Abstract：
UNASSIGNED: Theileriosis, caused by tick-borne hemoprotozoans of the genus Theileria, severely impacts the economics of the livestock industry in most tropical and subtropical countries. The aim of the present study was to detect Theileria spp. in domesticated animals (camels, cows, sheep, and goats) using direct microscopy and to determine the infection rate in geographically distinct regions in the northern emirates of the UAE.
UNASSIGNED: Blood samples (n = 536) were collected from clinically symptomatic and asymptomatic domesticated animals and subjected to Giemsa staining and examined microscopically for the identification of Theileria.
UNASSIGNED: Smears showed an overall rate of positivity for Theileria spp. in 325/536 (60.6%) animals. Different infection rates were recorded across the various animal groups in the different study areas (Middle region 215/386 [55.7%], East region 100/139 [71.9%]). Of the 11 goat samples collected from the North region, 10 (90%) were positive. Infection rates per animal group based on microscopy were as follows: camels, 3/35 (8.5%); cows, 19/36 (52.7%); goats, 200/303 (66%); and sheep, 103/162 (63.5%). Real-time polymerase chain reaction confirmation of all microscopy-positive samples identified 23/325 (7.1%) results as false-positive.
UNASSIGNED: This study clarified that Theileria spp. is present in the Middle (Sharjah, Umm Al Quwain, and Ajman), East, and North regions. This report also confirmed the use of direct microscopy with Giemsa-stained blood films as the method of choice for diagnosing acute infections. Further work is needed to molecularly determine the prevalence and species of Theileria spp. circulating in the different parts of the UAE.
UNASSIGNED: Blood samples (n = 536) were collected from clinically symptomatic and asymptomatic domesticated animals and subjected to Giemsa staining and examined microscopically for the identification of Theileria.
UNASSIGNED: Smears showed an overall rate of positivity for Theileria spp. in 325/536 (60.6%) animals. Different infection rates were recorded across the various animal groups in the different study areas (Middle region 215/386 [55.7%], East region 100/139 [71.9%]). Of the 11 goat samples collected from the North region, 10 (90%) were positive. Infection rates per animal group based on microscopy were as follows: camels, 3/35 (8.5%); cows, 19/36 (52.7%); goats, 200/303 (66%); and sheep, 103/162 (63.5%). Real-time polymerase chain reaction confirmation of all microscopy-positive samples identified 23/325 (7.1%) results as false-positive.
UNASSIGNED: This study clarified that Theileria spp. is present in the Middle (Sharjah, Umm Al Quwain, and Ajman), East, and North regions. This report also confirmed the use of direct microscopy with Giemsa-stained blood films as the method of choice for diagnosing acute infections. Further work is needed to molecularly determine the prevalence and species of Theileria spp. circulating in the different parts of the UAE.
摘要：
■Theileriosis,由Theileria属的tick传播的原生动物引起，严重影响大多数热带和亚热带国家的畜牧业经济。本研究的目的是检测Theileriaspp。在驯养的动物(骆驼，奶牛,绵羊,和山羊）使用直接显微镜并确定阿联酋北部酋长国地理上不同地区的感染率。
■从临床上有症状和无症状的驯养动物中收集血样（n=536），并进行Giemsa染色，并用显微镜检查以鉴定Theileria。
■涂片显示对Theileriaspp的总体阳性率。在325/536（60.6%）动物中。在不同研究区域的不同动物组中记录了不同的感染率（中部地区215/386[55.7％]，东部地区100/139[71.9%]）。在从北部地区收集的11只山羊样本中，10(90%)呈阳性。基于显微镜的每个动物组的感染率如下：骆驼，3/35（8.5%）；奶牛，19/36（52.7%）；山羊，200/303(66%)；和绵羊，103/162（63.5%）。所有显微镜阳性样品的实时聚合酶链反应确认23/325(7.1%)结果为假阳性。
■这项研究澄清了Theileriaspp。存在于中部(沙迦，嗯AlQuwain,和阿治曼)，东,和北部地区。该报告还证实了使用Giemsa染色的血膜直接显微镜作为诊断急性感染的首选方法。需要进一步的工作来从分子上确定Theileriaspp的患病率和种类。在阿联酋的不同地区流通。
■从临床上有症状和无症状的驯养动物中收集血样（n=536），并进行Giemsa染色，并用显微镜检查以鉴定Theileria。
■涂片显示对Theileriaspp的总体阳性率。在325/536（60.6%）动物中。在不同研究区域的不同动物组中记录了不同的感染率（中部地区215/386[55.7％]，东部地区100/139[71.9%]）。在从北部地区收集的11只山羊样本中，10(90%)呈阳性。基于显微镜的每个动物组的感染率如下：骆驼，3/35（8.5%）；奶牛，19/36（52.7%）；山羊，200/303(66%)；和绵羊，103/162（63.5%）。所有显微镜阳性样品的实时聚合酶链反应确认23/325(7.1%)结果为假阳性。
■这项研究澄清了Theileriaspp。存在于中部(沙迦，嗯AlQuwain,和阿治曼)，东,和北部地区。该报告还证实了使用Giemsa染色的血膜直接显微镜作为诊断急性感染的首选方法。需要进一步的工作来从分子上确定Theileriaspp的患病率和种类。在阿联酋的不同地区流通。
