PDF(Pubmed)
Abstract：
In this work, we present an ultra-low-cost smartphone device for in situ quantification of OP poisoning severity. The performance of the lens-less smartphone spectrum apparatus (LeSSA) is evaluated using standard human Interleukin-6 (IL-6) immunoassay kits. Upon dose-response curve fitting, LeSSA demonstrates an accuracy of 99.5%. The limit of detection (LOD) of LeSSA was evaluated through comparison of 6.4 pg/ml with standard laboratory grade UV-vis spectrophotometer at 5.5 pg/ml. Evaluating the capacity of LeSSA in spike solution by combining plasma cholinesterase (PChE) and human plasma shows consistency at agreement of 97.6% between LeSSA and the laboratory instrument. For application demonstration, the activity of PChE for 24 agricultural workers\' plasma samples was measured with LeSSA, showing exceptional agreement (r2 = 0.92) with the laboratory instrument reference. In addition to near laboratory grade accuracy, the total manufacturing cost of LeSSA is only $20 USD highlighting it\'s affordability. With LeSSA, clinicians can evaluate OP poisoning severity without the need to transport patient samples to facilities at far distances. Utilizing LeSSA, immediate results can be used for administration of appropriate treatment.
摘要：
在这项工作中,我们提出了一种超低成本的智能手机设备，用于原位定量OP中毒严重程度。使用标准人白细胞介素-6(IL-6)免疫测定试剂盒评估无透镜智能手机光谱装置(LeSSA)的性能。剂量反应曲线拟合后，LeSSA显示99.5%的准确度。LeSSA的检测限(LOD)通过在5.5pg/ml下与标准实验室级UV-vis分光光度计比较6.4pg/ml来评估。通过合并血浆胆碱酯酶（PChE）和人血浆来评估LeSSA在刺药溶液中的能力，LeSSA与实验室仪器之间的一致性为97.6％。对于应用演示，用LeSSA测量24名农业工人血浆样品的PChE活性，显示与实验室仪器参考的特殊一致性(r2=0.92)。除了接近实验室级的精度，LeSSA的总制造成本仅为20美元，突显了它的承受能力。有了LeSSA,临床医生可以评估OP中毒的严重程度，而无需将患者样本运送到远距离设施。利用LeSSA，即时结果可用于给予适当的治疗。
