Abstract：
The members of the GRAS gene family play important roles in regulating plant growth and development, but their functions in regulating early plant maturity traits are still unknown. In this study, we used a series of bioinformatics tools to identify GRAS gene family members and investigate the function of the gene family (GhGRAS55) using a genome-wide database of upland cotton samples. A total of 58 members of the GRAS gene family were identified and screened, which were distributed on 21 chromosomes within the whole cotton genome. The results of the phylogenetic analysis showed that the genes of upland cotton, island cotton, African cotton, Raymond cotton, and Arabidopsis were distributed in subfamilies I-VIII, although subfamily II did not contain any upland cotton or Arabidopsis GRAS family members. The structures and other characteristics of the genes in this family were clarified using bioinformatics technology. The transcriptomic sequencing results for early and late maturing cotton species showed that the expression of most GRAS family genes, such as GhGRAS10, GhGRAS5511, and GhGRAS55, was lower in early maturing species than late maturing species. We also found that cotton plants with GhGRAS55 genes that were silenced by virus-induced gene silencing (VIGS) technology showed early bud emergence phenotypes, so it could be speculated that the GhGRAS55 gene has the function of regulating early maturity in cotton.
摘要：
GRAS基因家族成员在调控植物生长发育中起着重要作用,但是它们在调节植物早期成熟性状方面的功能仍然未知。在这项研究中,我们使用一系列生物信息学工具来鉴定GRAS基因家族成员，并使用陆地棉样本的全基因组数据库研究基因家族(GhGRAS55)的功能。共鉴定并筛选了58个GRAS基因家族成员,分布在整个棉花基因组中的21条染色体上。系统发育分析结果表明,陆地棉的基因,岛棉,非洲棉花,雷蒙棉和拟南芥分布在I-VIII亚科中，尽管亚科II不包含任何陆地棉或拟南芥GRAS家族成员。利用生物信息学技术阐明了该家族基因的结构和其他特征。早熟和晚熟棉种的转录组测序结果表明,大多数GRAS家族基因的表达,例如GhGRAS10，GhGRAS5511和GhGRAS55，早熟物种低于晚熟物种。我们还发现，通过病毒诱导的基因沉默技术沉默的GhGRAS55基因的棉花植株表现出早期的芽出现表型，因此，可以推测GhGRAS55基因在棉花中具有调节早熟的功能。本文受版权保护。保留所有权利。
