Oxymatrine Alleviates Collagen-Induced Arthritis in Mice by Regulating the Immune Balance of T Cells.

氧化苦参碱通过调节 T 细胞免疫平衡减轻小鼠胶原性关节炎.

• 影响指数: 4.927
• 发表时间: 2023 Aug 4
• 来源期刊: Molecules
• DOI: 10.3390/molecules28155879
• PMID: 37570855
• 文章类型: Journal Article
• 中科院分区: Q3
• 作者列表: Cao G,Li J,Mao Z,Zhang Y

关键词: Th cell; gut microbiota; oxymatrine

Mesh: Mice; Animals; Arthritis, Experimental / metabolism; T-Lymphocytes, Regulatory; Arthritis, Rheumatoid / chemically induced drug therapy; Autoimmune Diseases / metabolism; Th17 Cells; Alkaloids / pharmacology metabolism; Cytokines / metabolism

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by systemic immunity and autoimmune disorders. We have previously found that oxymatrine (OMT), a natural alkaloid, can alleviate rheumatoid arthritis without knowing whether OMT can alleviate rheumatoid arthritis through gut microbiota. In this study, we found that OMT can alleviate collagen-induced arthritis in mice and reconstruct the immune balance of Th1/Th2, Treg/Th17, and Tfr/Tfh cells. Colon transcriptome gene enrichment analysis indicated that oxymatrine may alleviate collagen induced arthritis in mice through immune system process pathway. Furthermore, OMT significantly altered the gut microbiota variety, changed the composition of microbial colonies, and reshaped the gut microbiota of collagen-induced arthritis (CIA) mice, which may participate in the regulation of the balance of Th1/Th2, Treg/Th17, and Tfr/Tfh cells to alleviate collagen-induced arthritis in mice.

摘要

类风湿性关节炎（RA）是一种以全身免疫和自身免疫性疾病为特征的慢性自身免疫性疾病。我们以前发现氧化苦参碱(OMT)，一种天然生物碱,可以缓解类风湿性关节炎，而不知道OMT是否可以通过肠道菌群缓解类风湿性关节炎。在这项研究中,我们发现OMT可以减轻小鼠胶原性关节炎，并重建Th1/Th2，Treg/Th17和Tfr/Tfh细胞的免疫平衡。结肠转录组基因富集分析表明，氧化苦参碱可能通过免疫系统过程途径减轻胶原诱导的小鼠关节炎。此外，OMT显著改变了肠道微生物群的多样性，改变了微生物菌落的组成，重塑了胶原诱导的关节炎（CIA）小鼠的肠道菌群，可能参与调节Th1/Th2、Treg/Th17和Tfr/Tfh细胞的平衡,减轻小鼠胶原性关节炎。