PDF(Pubmed)
Abstract：
Obesity is a major risk factor for a variety of diseases and contributes to chronic inflammation. Resveratrol is a naturally occurring antioxidant that can reduce adipogenesis. In this study, the antiadipogenic and anti-inflammatory activities of resveratrol-enriched rice were investigated in 3T3-L1 adipocyte cells. Cotreatment of dexamethasone and isobutylmethylxanthin upregulated adipogenic transcription factors and signaling pathways. Subsequent treatment of adipocytes with rice seed extracts suppressed the differentiation of 3T3-L1 by downregulating adipogenic transcription factors (peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α) and signaling pathways (extracellular signal-regulated kinase 1/2 and protein kinase B Akt), this was especially observed in cells treated with germinated resveratrol-enriched rice seed extract (DJ526_5). DJ526_5 treatment also markedly reduced lipid accumulation in the cells and expression of adipogenic genes. Lipopolysaccharide (LPS)-induced inflammatory cytokines (prostaglandin-endoperoxide synthase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6) decreased in cells treated with DJ526_5. Collectively, DJ526_5 exerts antiadipogenic effects by suppressing the expression of adipogenesis transcription factors. Moreover, DJ526_5 ameliorates anti-inflammatory effects in 3T3-L1 adipocytes by inhibiting the activation of phosphorylation NF-κB p65 and ERK ½ (MAPK). These results highlight the potential of resveratrol-enriched rice as an alternative obesity-reducing and anti-inflammatory agent.
摘要：
肥胖是多种疾病的主要危险因素,并导致慢性炎症。白藜芦醇是一种天然存在的抗氧化剂，可以减少脂肪生成。在这项研究中,在3T3-L1脂肪细胞中研究了富含白藜芦醇的水稻的抗脂肪生成和抗炎活性。地塞米松和异丁基甲基黄质共同调节成脂转录因子和信号通路。随后用水稻种子提取物处理脂肪细胞通过下调成脂转录因子（过氧化物酶体增殖物激活受体γ和CCAAT/增强子结合蛋白α）和信号通路（细胞外信号调节激酶1/2和蛋白激酶BAkt）来抑制3T3-L1的分化，这在用富含白藜芦醇的发芽水稻种子提取物(DJ526_5)处理的细胞中尤其观察到。DJ526_5处理还显著降低了细胞中的脂质积累和脂肪生成基因的表达。脂多糖（LPS）诱导的炎症细胞因子（前列腺素-内过氧化物合酶2（COX-2），肿瘤坏死因子（TNF）-α，白细胞介素（IL）-1β，和IL-6）在用DJ526_5处理的细胞中降低。总的来说，DJ526_5通过抑制脂肪生成转录因子的表达发挥抗脂肪生成作用。此外，DJ526_5通过抑制磷酸化NF-κBp65和ERK½（MAPK）的激活来改善3T3-L1脂肪细胞的抗炎作用。这些结果突出了富含白藜芦醇的大米作为替代的减少肥胖和抗炎剂的潜力。
