PDF(Pubmed)
Abstract：
The embryo produced by in vitro oocyte maturation, fertilization, and embryonic development is an important resource for genetic improvement and has the potential to improve female fertility and to be programmed to produce offspring with superior ability for health and production. The cultured embryo is also an important component of several realized and potential technologies such as gene editing, somatic cell nuclear cloning, stem cell technologies and gamete generation in vitro. Full realization of the opportunities afforded by the in vitro-produced embryo will require overcoming some technical obstacles to cost-effective implementation of an embryo transfer program. Among the research goals for improving the penetration of embryo transfer in the cattle industry are development of methods to increase the supply of oocytes from genetically elite females, enhance the proportion of oocytes that become transferrable embryos, improve the fraction of embryos that establish pregnancy after transfer, reduce pregnancy wastage after pregnancy diagnosis, and identify culture conditions to optimize postnatal phenotype.
摘要：
体外卵母细胞成熟产生的胚胎，受精,胚胎发育是遗传改良的重要资源，有可能提高雌性生育能力，并被编程产生具有卓越健康和生产能力的后代。培养胚胎也是基因编辑等几种已实现和潜在技术的重要组成部分，体细胞核克隆,干细胞技术和配子体外生成。充分实现体外产生的胚胎所提供的机会将需要克服一些技术障碍以成本有效地实施胚胎移植计划。提高胚胎移植在养牛业中的渗透率的研究目标之一是开发增加遗传精英雌性卵母细胞供应的方法，提高成为可转移胚胎的卵母细胞的比例，改善转移后建立妊娠的胚胎比例，减少妊娠诊断后的妊娠浪费，并确定培养条件以优化产后表型。
