Abstract：
BACKGROUND: The aim of this study was to identify Enterocytozoon bieneusi and Encephalitozoon spp. in fecal samples of HIV + /AIDS and cancer patients undergoing chemotherapy, and comparing the results to healthy individuals in Mazandaran province, north of Iran.
METHODS: Stool samples were collected from 50 HIV + /AIDS patients, 50 cancer patients, and 50 healthy samples referred to medical centers in north of Iran. Stool samples were kept in 2.5% potassium dichromate at 4 °C, and stained by modified trichrome for light microscopy examination. The multiplex/nested-PCR targeted the small subunit ribosomal RNA (SSU rRNA) gene. To characterize genotypes, the nested PCR products sequenced by Bioneer Company and was subjected to phylogenetic analyses.
RESULTS: Ten of 50 samples (20%) of HIV + /AIDS patients, 5 of 50 samples (10%) of cancer patients, and 1 of healthy individuals (2%) were microscopically positive. From 50 HIV + / AIDS patients, E. bieneusi and Encephalitozoon spp. were detected in 10 (20%) and 6 (12%) cases, respectively. Furthermore, among cancer patients, 7 (14%) and 2 (4%) cases were E. bieneusi and Encephalitozoon spp., respectively. Out of 50 samples of healthy individuals, only 3 (6%) cases of E. bieneusi were observed. The genotypes D and M were detected among positive samples of E. bieneusi.
CONCLUSIONS: E. bieneusi and then Encephalitozoon spp. are common intestinal microsporidia in HIV + /AIDS patients and cancer patients undergoing chemotherapy in Mazandaran province. E. bieneusi genotype D seems to be the predominant genotype in Mazandaran province. Due to the considerable prevalence of intestinal microsporidia, physicians are advised to pay more attention to this opportunistic infection in high-risk groups.
METHODS: Stool samples were collected from 50 HIV + /AIDS patients, 50 cancer patients, and 50 healthy samples referred to medical centers in north of Iran. Stool samples were kept in 2.5% potassium dichromate at 4 °C, and stained by modified trichrome for light microscopy examination. The multiplex/nested-PCR targeted the small subunit ribosomal RNA (SSU rRNA) gene. To characterize genotypes, the nested PCR products sequenced by Bioneer Company and was subjected to phylogenetic analyses.
RESULTS: Ten of 50 samples (20%) of HIV + /AIDS patients, 5 of 50 samples (10%) of cancer patients, and 1 of healthy individuals (2%) were microscopically positive. From 50 HIV + / AIDS patients, E. bieneusi and Encephalitozoon spp. were detected in 10 (20%) and 6 (12%) cases, respectively. Furthermore, among cancer patients, 7 (14%) and 2 (4%) cases were E. bieneusi and Encephalitozoon spp., respectively. Out of 50 samples of healthy individuals, only 3 (6%) cases of E. bieneusi were observed. The genotypes D and M were detected among positive samples of E. bieneusi.
CONCLUSIONS: E. bieneusi and then Encephalitozoon spp. are common intestinal microsporidia in HIV + /AIDS patients and cancer patients undergoing chemotherapy in Mazandaran province. E. bieneusi genotype D seems to be the predominant genotype in Mazandaran province. Due to the considerable prevalence of intestinal microsporidia, physicians are advised to pay more attention to this opportunistic infection in high-risk groups.
摘要：
背景：这项研究的目的是鉴定肠孢子虫和头孢菌素。在HIV+/AIDS和接受化疗的癌症患者的粪便样本中，并将结果与Mazandaran省的健康个体进行比较，伊朗北部。
方法：收集50名HIV+/AIDS患者的粪便样本，50名癌症患者，和50个健康样本转诊到伊朗北部的医疗中心。粪便样品在4°C下保存在2.5％重铬酸钾中，用改良三色染色进行光学显微镜检查。多重/巢式PCR靶向小亚基核糖体RNA(SSUrRNA)基因。为了表征基因型，巢式PCR产物由Bioneer公司测序，并进行系统发育分析。
结果：50个HIV+/AIDS患者样本中有10个（20%），50个癌症患者样本中的5个(10%)，1名健康个体（2％）在显微镜下呈阳性。来自50名艾滋病毒/艾滋病患者，E.bieneusi和头孢菌素属。在10例(20%)和6例(12%)中检测到，分别。此外，在癌症患者中，7例（14％）和2例（4％）分别为E.bieneusi和头孢菌素。,分别。在50个健康个体样本中，仅观察到3例（6％）E.bieneusi。在E.bieneusi阳性样本中检测到基因型D和M。
结论：E.Bieneusi，然后是头孢菌素。是在Mazandaran省接受化疗的HIV/AIDS患者和癌症患者中常见的肠道微孢子虫。E.bieneusi基因型D似乎是Mazandaran省的主要基因型。由于肠道微孢子虫的患病率相当高，建议医生在高危人群中更多关注这种机会性感染.
方法：收集50名HIV+/AIDS患者的粪便样本，50名癌症患者，和50个健康样本转诊到伊朗北部的医疗中心。粪便样品在4°C下保存在2.5％重铬酸钾中，用改良三色染色进行光学显微镜检查。多重/巢式PCR靶向小亚基核糖体RNA(SSUrRNA)基因。为了表征基因型，巢式PCR产物由Bioneer公司测序，并进行系统发育分析。
结果：50个HIV+/AIDS患者样本中有10个（20%），50个癌症患者样本中的5个(10%)，1名健康个体（2％）在显微镜下呈阳性。来自50名艾滋病毒/艾滋病患者，E.bieneusi和头孢菌素属。在10例(20%)和6例(12%)中检测到，分别。此外，在癌症患者中，7例（14％）和2例（4％）分别为E.bieneusi和头孢菌素。,分别。在50个健康个体样本中，仅观察到3例（6％）E.bieneusi。在E.bieneusi阳性样本中检测到基因型D和M。
结论：E.Bieneusi，然后是头孢菌素。是在Mazandaran省接受化疗的HIV/AIDS患者和癌症患者中常见的肠道微孢子虫。E.bieneusi基因型D似乎是Mazandaran省的主要基因型。由于肠道微孢子虫的患病率相当高，建议医生在高危人群中更多关注这种机会性感染.
