Abstract：
Adult T cell leukemia/lymphoma is a malignancy with a poor prognosis caused by human T lymphocyte virus type 1 (HTLV-1) infection. Tax and HBZ are two major viral proteins that may be involved in oncogenesis by disrupting apoptosis. Because Bcl-xL plays an integral role in the anti-apoptotic pathway, this study examines the interaction between host apoptosis and oncoproteins. We investigated 37 HTLV-1-infected individuals, including 18 asymptomatic and 19 adult T cell leukemia/lymphoma (ATLL) subjects. mRNA was extracted and converted to cDNA from peripheral blood mononuclear cells, and then gene expression was determined using TaqMan q-PCR. Moreover, the HTLV-1 proviral load (PVL) was also measured using a commercial absolute quantification kit (Novin Gene, Iran). Data analysis revealed that the mean of TAX, HBZ, and PVL was significantly higher among the study groups (ATLL and carrier groups p = .003, p = .000, and p = .002 respectively). There was no statistical difference in Bcl-xL gene expression between the study groups (p = .323). It is proposed that this anti-apoptotic pathway may not be directly involved in the development of ATLL lymphoma. Bcl-xL, TAX, HBZ gene expression, and PVL can be utilized as prognostic markers.
摘要：
背景：成人T细胞白血病/淋巴瘤是一种由HTLV-1感染引起的预后不良的恶性肿瘤。TAX和HBZ是两种主要的病毒蛋白，可能通过破坏细胞凋亡而参与肿瘤发生。因为Bcl-xL在抗凋亡途径中起着不可或缺的作用，本研究探讨了宿主细胞凋亡与癌蛋白之间的相互作用。
方法：我们调查了37名HTLV-1感染者，包括18名无症状和19名ATLL受试者。从外周血单核细胞（PBMC）中提取mRNA并转化为cDNA，然后使用TaqManqPCR测定基因表达。此外，还使用商业绝对定量试剂盒(NovinGene，伊朗)。
结果：数据分析显示，HBZ,和PVL在研究组中显著更高(ATLL和携带者组P=(0.003)，P=(0.000)，和P=（0.002）分别）。各组Bcl-xL基因表达差异无统计学意义(P=0.323)。
结论：有人提出这种抗凋亡途径可能不直接参与ATLL淋巴瘤的发展。Tax,HBZ基因表达,和PVL可以用作预后标志物。
方法：我们调查了37名HTLV-1感染者，包括18名无症状和19名ATLL受试者。从外周血单核细胞（PBMC）中提取mRNA并转化为cDNA，然后使用TaqManqPCR测定基因表达。此外，还使用商业绝对定量试剂盒(NovinGene，伊朗)。
结果：数据分析显示，HBZ,和PVL在研究组中显著更高(ATLL和携带者组P=(0.003)，P=(0.000)，和P=（0.002）分别）。各组Bcl-xL基因表达差异无统计学意义(P=0.323)。
结论：有人提出这种抗凋亡途径可能不直接参与ATLL淋巴瘤的发展。Tax,HBZ基因表达,和PVL可以用作预后标志物。
