PDF(Pubmed)
Abstract：
OBJECTIVE: The aim of this systematic review was to investigate tendon-specific microRNAs (miRNAs) as biomarkers for the detection of tendinopathies or degenerative tendon ruptures. Also, their regulatory mechanisms within the tendon pathophysiology were summarized.
METHODS: A systematic literature research was performed using the PRISMA guidelines. The search was conducted in the Pubmed database. The SIGN checklist was used to assess the study quality of the included original studies. To determine the evidence and direction of the miRNA expression rates, a best-evidence synthesis was carried out, whereby only studies with at least a borderline methodological quality were considered for validity purposes.
RESULTS: Three thousand three hundred seventy studies were reviewed from which 22 fulfilled the inclusion criteria. Moderate evidence was found for miR-140-3p and miR-425-5p as potential biomarkers for tendinopathies as well as for miR-25-3p, miR-29a-3p, miR-140-3p, and miR-425-5p for the detection of degenerative tendon ruptures. This evidence applies to tendons at the upper extremity in elderly patients. All miRNAs were associated with inflammatory cytokines as interleukin-6 or interleukin-1ß and tumor necrosis factor alpha.
CONCLUSIONS: Moderate evidence exists for four miRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients. The identified miRNAs are associated with inflammatory processes.
METHODS: A systematic literature research was performed using the PRISMA guidelines. The search was conducted in the Pubmed database. The SIGN checklist was used to assess the study quality of the included original studies. To determine the evidence and direction of the miRNA expression rates, a best-evidence synthesis was carried out, whereby only studies with at least a borderline methodological quality were considered for validity purposes.
RESULTS: Three thousand three hundred seventy studies were reviewed from which 22 fulfilled the inclusion criteria. Moderate evidence was found for miR-140-3p and miR-425-5p as potential biomarkers for tendinopathies as well as for miR-25-3p, miR-29a-3p, miR-140-3p, and miR-425-5p for the detection of degenerative tendon ruptures. This evidence applies to tendons at the upper extremity in elderly patients. All miRNAs were associated with inflammatory cytokines as interleukin-6 or interleukin-1ß and tumor necrosis factor alpha.
CONCLUSIONS: Moderate evidence exists for four miRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients. The identified miRNAs are associated with inflammatory processes.
摘要：
目的：本系统综述的目的是研究肌腱特异性microRNAs（miRNAs）作为检测肌腱病变或退行性肌腱断裂的生物标志物。此外，总结了它们在肌腱病理生理学中的调控机制。
方法：使用PRISMA指南进行了系统的文献研究。在Pubmed数据库中进行搜索。SIGN检查表用于评估纳入的原始研究的研究质量。为了确定miRNA表达率的证据和方向，进行了最佳证据综合，因此，出于有效性目的，仅考虑具有至少临界方法学质量的研究。
结果：回顾了三千三百七十项研究，其中22项符合纳入标准。发现了miR-140-3p和miR-425-5p作为肌腱病以及miR-25-3p的潜在生物标志物的中等证据。miR-29a-3p,miR-140-3p,和miR-425-5p用于检测退行性肌腱断裂。该证据适用于老年患者的上肢肌腱。所有miRNA均与炎性细胞因子如白细胞介素-6或白细胞介素-1β和肿瘤坏死因子α相关。
结论：4种miRNAs作为老年患者上肢肌腱病变和退行性肌腱断裂的潜在生物标志物存在中等证据。鉴定的miRNA与炎症过程相关。
方法：使用PRISMA指南进行了系统的文献研究。在Pubmed数据库中进行搜索。SIGN检查表用于评估纳入的原始研究的研究质量。为了确定miRNA表达率的证据和方向，进行了最佳证据综合，因此，出于有效性目的，仅考虑具有至少临界方法学质量的研究。
结果：回顾了三千三百七十项研究，其中22项符合纳入标准。发现了miR-140-3p和miR-425-5p作为肌腱病以及miR-25-3p的潜在生物标志物的中等证据。miR-29a-3p,miR-140-3p,和miR-425-5p用于检测退行性肌腱断裂。该证据适用于老年患者的上肢肌腱。所有miRNA均与炎性细胞因子如白细胞介素-6或白细胞介素-1β和肿瘤坏死因子α相关。
结论：4种miRNAs作为老年患者上肢肌腱病变和退行性肌腱断裂的潜在生物标志物存在中等证据。鉴定的miRNA与炎症过程相关。
