PDF(Pubmed)
Abstract：
Brucellosis is a common zoonosis, which is caused by Brucella infection, and Brucella often infects livestock, leading to abortion and infertility. At present, human brucellosis remains one of the major public health problems in China. According to previous research, most areas in northwest China, including Xinjiang, Tibet, and other regions, are severely affected by Brucella. Although there are vaccines against animal Brucellosis, the effect is often poor. In addition, there is no corresponding vaccine for human Brucellosis infection. Therefore, a new strategy for early prevention and treatment of Brucella is needed. A multi-epitope vaccine should be developed. In this study, we identified the antigenic epitopes of the Brucella type IV secretion system VirB8 and Virb10 using an immunoinformatics approach, and screened out 2 cytotoxic T lymphocyte (CTL) epitopes, 9 helper T lymphocyte (HTL) epitopes, 6 linear B cell epitopes, and 6 conformational B cell epitopes. These advantageous epitopes are spliced together through different linkers to construct a multi-epitope vaccine. The silico tests showed that the multi-epitope vaccine was non-allergenic and had a strong interaction with TLR4 molecular docking. In immune simulation results, the vaccine construct may be useful in helping brucellosis patients to initiate cellular and humoral immunity. Overall, our findings indicated that the multi-epitope vaccine construct has a high-quality structure and suitable characteristics, which may provide a theoretical basis for the development of a Brucella vaccine.
摘要：
布鲁氏菌病是一种常见的人畜共患病，这是由布鲁氏菌感染引起的，布鲁氏菌经常感染牲畜，导致流产和不孕。目前,人类布鲁氏菌病仍然是中国主要的公共卫生问题之一。根据之前的研究,中国西北地区的大部分地区，包括新疆,西藏,和其他地区,受到布鲁氏菌的严重影响.虽然有针对动物布鲁氏菌病的疫苗，效果往往很差。此外,目前尚无相应的针对人类布鲁氏菌病感染的疫苗。因此,需要一种新的策略来早期预防和治疗布鲁氏菌。应该开发多表位疫苗。在这项研究中,我们使用免疫信息学方法鉴定了布鲁氏菌IV型分泌系统VirB8和Virb10的抗原表位，筛选出2个细胞毒性T淋巴细胞(CTL)表位,9辅助性T淋巴细胞（HTL）表位，6个线性B细胞表位，和6个构象B细胞表位。这些有利的表位通过不同的接头剪接在一起以构建多表位疫苗。实验结果表明,该多表位疫苗无变应原性,与TLR4分子对接有较强的相互作用。在免疫模拟结果中，该疫苗构建体可能有助于布鲁氏菌病患者启动细胞和体液免疫。总的来说,我们的发现表明，多表位疫苗构建体具有高质量的结构和合适的特性，为布鲁氏菌疫苗的研制提供了理论依据。
