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Abstract：
Fascin (FSCN) is an actin-binding protein that serves a critical role in cell migration and invasion, contributing to tumor metastasis. However, there is little known about the function of FSCN family in kidney renal clear cell carcinoma (KIRC). The present study used the UALCAN, gene expression profiling interactive analysis, The Cancer Genome Atlas, cBioPortal, STRING and The Tumor Immune Estimation Resource databases to investigate the transcription level, genetic alteration and biological function of FSCNs in KIRC and their association with the prognosis value and immune cell infiltration in patients with KIRC. Results showed that the expression of FSCN1 and FSCN3 was markedly upregulated in patients with KIRC, while the expression of FSCN2 showed an opposite trend, which was the same as the experiments. Furthermore, the expression levels of FSCNs were associated with pathological stage, molecular subtypes and tumor grade. The expression levels of FSCNs were statistically correlated with the immune cell infiltration in KIRC. Higher expression levels of FSCN1 and FSCN3 were associated with worse overall survival (OS) and progression-free interval of patients bearing KIRC. Univariate and multivariate analysis demonstrated that FSCN2 was an independent risk factor for OS time in KIRC. Furthermore, mutations in FSCNs were significantly associated with poor OS and progression-free survival in patients with KIRC. The FSCNs were involved in pathways including focal adhesion, endocytosis, hypertrophic cardiomyopathy, regulation of actin cytoskeleton. The results indicated that FSCN2 might serve as an independent prognostic factor for OS of KIRC and that FSCN1 and FSCN3 can be used as favorable biomarkers for predicting clinical outcomes in KIRC.
摘要：
Fascin（FSCN）是一种肌动蛋白结合蛋白，在细胞迁移和侵袭中起关键作用。有助于肿瘤转移。然而,FSCN家族在肾透明细胞癌（KIRC）中的作用鲜为人知。本研究使用了UALCAN，基因表达谱交互式分析，癌症基因组图谱,cBioPortal,STRING和肿瘤免疫估计资源数据库，以调查转录水平，KIRC中FSCNs的遗传改变和生物学功能及其与KIRC患者预后价值和免疫细胞浸润的关系。结果显示，在KIRC患者中，FSCN1和FSCN3的表达明显上调，而FSCN2的表达呈现相反的趋势,和实验一样.此外，FSCNs的表达水平与病理分期有关,分子亚型和肿瘤分级。FSCNs的表达水平与KIRC中的免疫细胞浸润有统计学相关性。FSCN1和FSCN3的较高表达水平与携带KIRC的患者的较差总生存期(OS)和无进展间隔相关。单因素和多因素分析表明FSCN2是KIRC中OS时间的独立危险因素。此外，在KIRC患者中,FSCNs突变与不良OS和无进展生存期显著相关.FSCN参与了包括粘着斑在内的通路，内吞作用,肥厚型心肌病,肌动蛋白细胞骨架的调节。结果表明，FSCN2可能是KIRCOS的独立预后因素，FSCN1和FSCN3可作为预测KIRC临床结局的有利生物标志物。
