PDF(Pubmed)
Abstract：
Head-fixed behavioral experiments in rodents permit unparalleled experimental control, precise measurement of behavior, and concurrent modulation and measurement of neural activity. Here, we present OHRBETS (Open-Source Head-fixed Rodent Behavioral Experimental Training System; pronounced \'Orbitz\'), a low-cost, open-source platform of hardware and software to flexibly pursue the neural basis of a variety of motivated behaviors. Head-fixed mice tested with OHRBETS displayed operant conditioning for caloric reward that replicates core behavioral phenotypes observed during freely moving conditions. OHRBETS also permits optogenetic intracranial self-stimulation under positive or negative operant conditioning procedures and real-time place preference behavior, like that observed in freely moving assays. In a multi-spout brief-access consumption task, mice displayed licking as a function of concentration of sucrose, quinine, and sodium chloride, with licking modulated by homeostatic or circadian influences. Finally, to highlight the functionality of OHRBETS, we measured mesolimbic dopamine signals during the multi-spout brief-access task that display strong correlations with relative solution value and magnitude of consumption. All designs, programs, and instructions are provided freely online. This customizable platform enables replicable operant and consummatory behaviors and can be incorporated with methods to perturb and record neural dynamics in vivo.
摘要：
啮齿动物的头部固定行为实验允许无与伦比的实验控制，行为的精确测量，同时调节和测量神经活动。在这里，我们介绍OHRBETS（开源头部固定啮齿动物行为实验训练系统；发音为“Orbitz”），一个低成本的，硬件和软件的开源平台，以灵活地追求各种动机行为的神经基础。用OHRBETS测试的头部固定小鼠显示出热量奖励的操作性调节，可复制在自由移动条件下观察到的核心行为表型。OHRBETS还允许在阳性或阴性操作条件程序和实时位置偏好行为下进行光遗传学颅内自我刺激，就像在自由移动的化验中观察到的那样。在多spout短暂访问消费任务中，小鼠表现出舔蔗糖浓度的函数，奎宁,和氯化钠，通过体内平衡或昼夜节律的影响来调节舔。最后,为了突出OHRBETS的功能，我们在多喷口短暂访问任务中测量了中脑边缘多巴胺信号，该信号与相对解值和消耗幅度具有很强的相关性。所有的设计,programs,和说明在网上免费提供。这种可定制的平台能够实现可复制的操作和完善的行为，并且可以与干扰和记录体内神经动力学的方法结合在一起。
